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聚乙烯醇/丝素蛋白/银纳米粒子复合纳米纤维作为MG-63细胞生长的基质

Poly(vinyl alcohol)/Silk Fibroin/Ag-NPs Composite Nanofibers as a Substrate for MG-63 Cells' Growth.

作者信息

Mejía Suaza Monica L, Leos Rivera Jennifer C, Rodríguez Padilla Maria C, Moncada Acevedo Maria E, Ossa Orozco Claudia P, Zarate Triviño Diana G

机构信息

Advanced Materials and Energy (MATyER) Research Group, Faculty of Engineering, Metropolitan Technological Institute (ITM), Medellin 050012, Colombia.

Laboratory of Immunology and Virology, Faculty of Biological Sciences, Autonomous University of Nuevo Leon, Monterrey 64000, Mexico.

出版信息

Polymers (Basel). 2023 Apr 11;15(8):1838. doi: 10.3390/polym15081838.

DOI:10.3390/polym15081838
PMID:37111985
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10144439/
Abstract

Nanofiber scaffolds of polyvinyl alcohol, silk fibroin from cocoons, and silver nanoparticles were developed as a substrate for MG-63 growth. The fiber morphology, mechanical properties, thermal degradation, chemical composition, and water contact angle were investigated. In vitro tests were performed by the cell viability MTS test of MG-63 cells on electrospun PVA scaffolds, mineralization was analyzed by alizarin red, and the alkaline phosphatase (ALP) assay was evaluated. At higher PVA concentrations, Young's modulus (E) increased. The addition of fibroin and silver nanoparticles improved the thermal stability of PVA scaffolds. FTIR spectra indicated characteristic absorption peaks related to the chemical structures of PVA, fibroin, and Ag-NPs, demonstrating good interactions between them. The contact angle of the PVA scaffolds decreased with the incorporation of fibroin and showed hydrophilic characteristics. In all concentrations, MG-63 cells on PVA/fibroin/Ag-NPs scaffolds had higher cell viability than PVA pristine. On day ten of culture, PVA18/SF/Ag-NPs showed the highest mineralization, observed by the alizarin red test. PVA10/SF/Ag-NPs presented the highest alkaline phosphatase activity after an incubation time of 37 h. The achievements indicate the potential of the nanofibers of PVA18/SF/Ag-NPs as a possible substitute for bone tissue engineering (BTE).

摘要

开发了由聚乙烯醇、蚕茧丝素蛋白和银纳米颗粒组成的纳米纤维支架作为MG-63细胞生长的基质。研究了纤维形态、力学性能、热降解、化学成分和水接触角。通过对静电纺丝PVA支架上的MG-63细胞进行细胞活力MTS测试进行体外试验，用茜素红分析矿化情况，并评估碱性磷酸酶（ALP）活性。在较高的PVA浓度下，杨氏模量（E）增加。丝素蛋白和银纳米颗粒的加入提高了PVA支架的热稳定性。傅里叶变换红外光谱表明了与PVA、丝素蛋白和银纳米颗粒化学结构相关的特征吸收峰，证明了它们之间良好的相互作用。PVA支架的接触角随着丝素蛋白的加入而降低，并表现出亲水性特征。在所有浓度下，PVA/丝素蛋白/银纳米颗粒支架上的MG-63细胞比原始PVA具有更高的细胞活力。在培养的第10天，通过茜素红测试观察到PVA18/丝素蛋白/银纳米颗粒表现出最高的矿化程度。在孵育37小时后，PVA10/丝素蛋白/银纳米颗粒呈现出最高的碱性磷酸酶活性。这些成果表明PVA18/丝素蛋白/银纳米颗粒纳米纤维作为骨组织工程（BTE）可能替代品的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d741/10144439/9503f33c20e4/polymers-15-01838-g011.jpg
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