Annu Int Conf IEEE Eng Med Biol Soc. 2021 Nov;2021:3105-3108. doi: 10.1109/EMBC46164.2021.9630341.
The Interstitial Cells of Cajal (ICC) are specialized gastrointestinal (GI) pacemaker cells that generate and actively propagate electrophysiological events called slow waves. Slow waves regulate the GI motility necessary for digestion. Several functional GI motility disorders have been associated with depletion in the ICC. In this study, a validated Fast Random Forest (FRF) classification method using Trainable WEKA Segmentation for segmenting the networks of ICC was applied to confocal microscopy images of a whole mount tissue from the distal antrum of a mouse stomach (583 × 3,376 × 133 μm, parcellated into 24 equal image stacks). The FRF model performance was compared to 6 manually segmented subflelds and produced an area under the receiver-operating characteristic (AUROC) of 0.95. Structural variations of ICC network in the longitudinal muscle (ICC-LM) and myenteric plexus (ICC-MP) were quantified. The average volume of ICC-MP was significantly higher than ICC-LM at any point throughout the antral tissue sampled. There was a pronounced decline of up to 80% in ICC-LM (from 3,705 μm to 716 μm) over a distance of 279.3 μm, that eventually diminished towards the distal antrum. However, an inverse relationship was observed in ICC-MP with an overall increase of up to 157% (from 59,100 μm to 151,830 μm) over a distance of approximately 2 mm that proceeds towards the distal antrum.
Cajal 间质细胞（ICC）是专门的胃肠道（GI）起搏细胞，可产生并主动传播称为慢波的电生理事件。慢波调节消化所需的 GI 运动。几种功能性 GI 运动障碍与 ICC 的耗竭有关。在这项研究中，使用经过验证的 Fast Random Forest（FRF）分类方法，该方法使用可训练的 WEKA 分段对来自小鼠胃远端窦部的整个组织的共焦显微镜图像（583×3,376×133μm，分为 24 个相等的图像堆栈）进行分段。将 FRF 模型性能与 6 个手动分段子字段进行了比较，并产生了 0.95 的接收器操作特性（AUROC）。定量了 ICC 网络在纵行肌（ICC-LM）和肌间神经丛（ICC-MP）中的结构变化。在整个取样窦组织中，ICC-MP 的平均体积始终高于 ICC-LM。在 279.3μm 的距离内，ICC-LM 下降了高达 80％（从 3705μm 到 716μm），最终在远端窦部消失。但是，在 ICC-MP 中观察到了相反的关系，总体上增加了高达 157％（从 59,100μm 到 151,830μm），在大约 2mm 的距离内，这种关系一直持续到远端窦部。
