Chakraborty B N, Chakraborty U, Basu K
Cellular, Molecular and Microbiology Division, Department of Biological Sciences, University of Calgary, Alberta, Canada.
Canola Research Centre, Alberta Wheat Pool, Calgary, Alberta, Canada.
Lett Appl Microbiol. 1994 Feb;18(2):74-76. doi: 10.1111/j.1472-765X.1994.tb00807.x.
Phyllosphere micro-organisms of Brassica napus were isolated and their antagonism against Leptosphaeria maculans, causal agent of blackleg disease, was tested in vitro. In paired culture, Erwinia herbicola was found to be highly antagonistic to L. maculans. Bioassay of the culture filtrate of the bacterium against the test fungus revealed that Erw. herbicola secretes an antifungal substance into the culture medium. This substance was partially thermolabile and markedly reduced the germination and germ tube length of L. maculans. Aqueous bacterial suspensions and cold-sterilized culture filtrates, when applied to the seedlings prior to inoculation, significantly reduced the severity of blackleg disease.
对甘蓝型油菜叶际微生物进行了分离，并在体外测试了它们对黑胫病病原菌——大茎点菌的拮抗作用。在配对培养中，发现草生欧文氏菌对大茎点菌具有高度拮抗作用。该细菌培养滤液对测试真菌的生物测定表明，草生欧文氏菌会向培养基中分泌一种抗真菌物质。这种物质部分不耐热，显著降低了大茎点菌的萌发率和芽管长度。在接种前将细菌水悬浮液和冷灭菌培养滤液施用于幼苗时，可显著降低黑胫病的严重程度。
