Weusten J J, Blankenstein M A, Gmelig-Meyling F H, Schuurman H J, Kater L, Thijssen J H
Acta Endocrinol (Copenh). 1986 Jul;112(3):409-14. doi: 10.1530/acta.0.1120409.
We studied oestrogen binding sites in blood mononuclear cells from healthy blood donors, patients with leukaemia or systemic lupus erythematosus, and in thymocytes, using the dextran-coated charcoal assay and Scatchard analysis of binding data. Using 3H-labelled oestradiol as ligand, inaccurate results were obtained which could be related to the low amounts of binding sites. Using 125I-labelled ligand, saturable oestradiol binding sites could be demonstrated in low amount (mean value 2.1 fmol/mg of cytosolic protein) and high affinity (mean Kd value 0.26 nM; mean Ka value 3.85 X 10(9) M-1). The binding could be inhibited by unlabelled oestradiol but not with oestrone, dihydrotestosterone, cortisol and the progestin-receptor ligand Org 2058. We conclude that blood mononuclear cells and thymocytes contain true oestrogen receptors. This conclusion supports current hypotheses on the involvement of such receptors in oestrogen-mediated modulation of the immune system.
我们使用葡聚糖包被活性炭分析法和结合数据的Scatchard分析，研究了健康献血者、白血病患者或系统性红斑狼疮患者血液中的单核细胞以及胸腺细胞中的雌激素结合位点。以3H标记的雌二醇作为配体时，得到的结果不准确，这可能与结合位点数量少有关。使用125I标记的配体时，可证明存在少量（平均2.1 fmol/mg胞质蛋白）且具有高亲和力（平均Kd值0.26 nM；平均Ka值3.85×10(9) M-1）的可饱和雌二醇结合位点。未标记的雌二醇可抑制这种结合，但雌酮、双氢睾酮、皮质醇和孕激素受体配体Org 2058则不能。我们得出结论，血液单核细胞和胸腺细胞含有真正的雌激素受体。这一结论支持了当前关于此类受体参与雌激素介导的免疫系统调节的假说。
