Moriconi Débora E, Macedo-Lopes Catarina, Sartorio Adriana, Juárez M Patricia, Girotti Juan R, Calderón-Fernández Gustavo M
Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), Facultad de Ciencias Médicas, La Plata, Argentina.
Laboratório Interdisciplinar de Vigilância Entomológica em Diptera e Hemiptera, Instituto Oswaldo Cruz, Manguinhos, Rio de Janeiro, Brazil.
J Med Entomol. 2022 Mar 16;59(2):554-564. doi: 10.1093/jme/tjab199.
The Triatoma sordida subcomplex traditionally included four triatomine species, T. sordida, Triatoma garciabesi, Triatoma guasayana, and Triatoma patagonica, distributed in the Southern Cone of South America. These species have a large intraspecific variability together with an overall similarity, making difficult to establish their taxonomic status. Many cytogenetic, morphometric, and molecular markers have been applied to address this. Recent studies have posed concerns on the inclusion of T. guasayana and T. patagonica within the subcomplex. Also, T. sordida from Argentina has been designed as a new species, Triatoma rosai. Using the cuticular hydrocarbon pattern as chemotaxonomic marker, the relationships among several populations of these species were analyzed by capillary gas chromatography and linear discriminant analysis along 25 collection sites in Argentina, Bolivia, Brazil, and Paraguay. T. sordida and T. rosai populations were differentially clustered in two CHC-based groups: "Group 1" included T. sordida from Eastern Brazil, Eastern Paraguay, and the Bolivian populations from La Paz and Izozog G1; "Group 2" included T. rosai, and T. sordida from Izozog G2 (Bolivia), and Western Paraguay. Whereas T. garciabesi remained closely related to T. sordida and T. rosai, T. guasayana, and T. patagonica were clearly separated from the species of the T. sordida subcomplex. Our results agree with those from other several techniques suggesting that the taxonomy of the T. sordida subcomplex should be revised.
污色锥猎蝽亚复合体传统上包括四种锥猎蝽，即污色锥猎蝽、加西亚贝锥猎蝽、瓜萨亚纳锥猎蝽和巴塔哥尼亚锥猎蝽，分布于南美洲的南锥体地区。这些物种种内变异大但整体相似，难以确定它们的分类地位。许多细胞遗传学、形态测量学和分子标记已被用于解决这一问题。最近的研究对瓜萨亚纳锥猎蝽和巴塔哥尼亚锥猎蝽被纳入该亚复合体提出了质疑。此外，来自阿根廷的污色锥猎蝽已被定为一个新物种，即罗萨锥猎蝽。以表皮碳氢化合物模式作为化学分类标记，通过毛细管气相色谱法和线性判别分析，对阿根廷、玻利维亚、巴西和巴拉圭25个采集地点的这些物种的多个种群之间的关系进行了分析。污色锥猎蝽和罗萨锥猎蝽种群在基于表皮碳氢化合物的两个组中差异聚类：“第1组”包括来自巴西东部、巴拉圭东部以及来自拉巴斯和伊佐佐格G1的玻利维亚种群的污色锥猎蝽；“第2组”包括罗萨锥猎蝽，以及来自伊佐佐格G2（玻利维亚）和巴拉圭西部的污色锥猎蝽。加西亚贝锥猎蝽与污色锥猎蝽和罗萨锥猎蝽关系密切，而瓜萨亚纳锥猎蝽和巴塔哥尼亚锥猎蝽则明显与污色锥猎蝽亚复合体的物种分开。我们的结果与其他几种技术的结果一致，表明污色锥猎蝽亚复合体的分类应予以修订。
