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一种用于简便高效多重无转导基因编辑的案例工具包。

A CASE toolkit for easy and efficient multiplex transgene-free gene editing.

机构信息

Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.

College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.

出版信息

Plant Physiol. 2022 Mar 28;188(4):1843-1847. doi: 10.1093/plphys/kiab573.

DOI:10.1093/plphys/kiab573
PMID:34893900
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8968415/
Abstract

An integrated transgene-free multiplex gene-editing toolkit based on the Transgene Killer CRISPR technology greatly saves labor, time, and cost.

摘要

基于 Transgene Killer CRISPR 技术的整合型无转基因多重基因编辑工具大大节省了人力、时间和成本。

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本文引用的文献

1
Technological breakthroughs in generating transgene-free and genetically stable CRISPR-edited plants.生成无转基因且基因稳定的CRISPR编辑植物的技术突破。
aBIOTECH. 2019 Dec 3;1(1):88-96. doi: 10.1007/s42994-019-00013-x. eCollection 2020 Jan.
2
Pyramiding favorable alleles in an elite wheat variety in one generation by CRISPR-Cas9-mediated multiplex gene editing.通过CRISPR-Cas9介导的多重基因编辑在一代中聚合优良小麦品种中的有利等位基因。
Mol Plant. 2021 Jun 7;14(6):847-850. doi: 10.1016/j.molp.2021.03.024. Epub 2021 Apr 1.
3
Expanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems.利用 Cas12a 同源蛋白和高度可多重编辑系统扩展植物基因组工程的范围。
Nat Commun. 2021 Mar 29;12(1):1944. doi: 10.1038/s41467-021-22330-w.
4
Generation of a multiplex mutagenesis population via pooled CRISPR-Cas9 in soya bean.通过大豆中的 pooled CRISPR-Cas9 生成多路诱变文库。
Plant Biotechnol J. 2020 Mar;18(3):721-731. doi: 10.1111/pbi.13239. Epub 2019 Sep 9.
5
Applications of multiplex genome editing in higher plants.多基因编辑在高等植物中的应用。
Curr Opin Biotechnol. 2019 Oct;59:93-102. doi: 10.1016/j.copbio.2019.02.015. Epub 2019 Apr 10.
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