利用简化的 CRISPR-Cpf1 和 CRISPR-Cas9 系统在水稻中进行多重基因编辑。
Multiplex gene editing in rice with simplified CRISPR-Cpf1 and CRISPR-Cas9 systems.
机构信息
Shanghai Center for Plant Stress Biology and Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 201602, China.
Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USA.
出版信息
J Integr Plant Biol. 2018 Aug;60(8):626-631. doi: 10.1111/jipb.12667. Epub 2018 Jul 7.
We developed simplified single transcriptional unit (SSTU) CRISPR systems for multiplex gene editing in rice using FnCpf1, LbCpf1 or Cas9, in which the nuclease and its crRNA array are co-expressed from a single Pol II promoter, without any additional processing machinery. Our SSTU systems are easy to construct and effective in mediating multiplex genome editing.
我们开发了简化的单转录单元（SSTU）CRISPR 系统，用于使用 FnCpf1、LbCpf1 或 Cas9 在水稻中进行多重基因编辑，其中核酸酶及其 crRNA 阵列由单个 Pol II 启动子共同表达，而无需任何额外的加工机制。我们的 SSTU 系统易于构建，并且在介导多重基因组编辑方面非常有效。
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