Yang Tao, Xu Xin-Tong, Tang Li-Jun, Wei Wen-Tao, Zhao Yuan-Yuan, Liu Jin-Xin, Yao Xue-Feng, Zhao Heng, Liu Chun-Ming, Bai Ai-Ning
Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
Plants (Basel). 2025 Jan 7;14(2):151. doi: 10.3390/plants14020151.
Plant A/T-rich sequence- and zinc-binding protein (PLATZ) is a type of plant-specific zinc-dependent DNA-binding protein that binds to A/T-rich DNA sequences. This family is essential for plant growth, development, and stress response. In this study, 15 were identified in the rice genome with complete PLATZ-conserved domains by CD-search, similar to those found in angiosperms. Multi-species phylogenetic analysis showed that PLATZs were conserved in photosynthetic organisms, and an evolutionary branch unique to angiosperms was identified among members of the PLATZ family. Fifteen OsPLATZs were represented by five groups, each with distinct characteristics. An analysis of protein structures and sequence motifs showed that OsPLATZs were similar within groups, but varied between them. The expression profile and qRT-PCR results showed that had distinct expression patterns in different tissues, with some responding to stress induction. Most of the OsPLATZs localized to the nuclei, and were predicted to bind to DNA sequences by AlphaFold3, suggesting that they likely function as conventional transcription factors. We also identified , a caryopsis-specific gene that regulates grain filling and caryopsis development in rice. This research lays the foundation for exploring the structural diversity, evolutionary traits, expression profile, and possible roles of PLATZ transcription factors in rice.
植物富含A/T序列和锌结合蛋白（PLATZ）是一类植物特有的依赖锌的DNA结合蛋白，可与富含A/T的DNA序列结合。该家族对植物生长、发育和应激反应至关重要。在本研究中，通过CD-search在水稻基因组中鉴定出15个具有完整PLATZ保守结构域的蛋白，与被子植物中发现的类似。多物种系统发育分析表明，PLATZ在光合生物中保守，并且在PLATZ家族成员中鉴定出一个被子植物特有的进化分支。15个水稻PLATZ（OsPLATZ）分为五组，每组具有不同特征。蛋白质结构和序列基序分析表明，OsPLATZ在组内相似，但组间不同。表达谱和qRT-PCR结果表明，OsPLATZ在不同组织中具有不同的表达模式，有些对胁迫诱导有反应。大多数OsPLATZ定位于细胞核，并通过AlphaFold3预测可与DNA序列结合，表明它们可能作为传统转录因子发挥作用。我们还鉴定出一个颖果特异性基因，该基因调控水稻的籽粒灌浆和颖果发育。本研究为探索PLATZ转录因子在水稻中的结构多样性、进化特征、表达谱及可能作用奠定了基础。
