Golsanamlu Zahra, Soleymani Jafar, Gharekhani Afshin, Jouyban Abolghasem
Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Heliyon. 2023 Jul 20;9(8):e18404. doi: 10.1016/j.heliyon.2023.e18404. eCollection 2023 Aug.
Tacrolimus (Tac) is a well-documented immunosuppressive agent for the prevention of graft-vs-host diseases in several types of organ transplants. The narrow therapeutic window and the individual-variable pharmacokinetics of Tac demonstrate the importance of regular therapeutic drug monitoring (TDM) as an imperative concept for its oral medication regimens. A simple, one-step, selective, and sensitive colorimetric platform is fabricated for the determination of Tac by surface modification of the silver nanoparticles (AgNPs) via norepinephrine (NE) molecules. The attachment of NE and Tac induces the aggregation of the AgNPs, which is observed by color distinction (yellow to brown) and a noteworthy shifting of the absorption peak in the visible region. The fabricated nanoprobe can detect Tac concentrations in plasma samples in two linear ranges from 2 ng/mL to 70 ng/mL and 70 ng/mL to 1000 ng/mL with R > 0.99. The limit of detection (LOD) was calculated as low as 0.1 ng/mL. The developed method was applied for the determination of Tac in patient's plasma samples under Tac medication therapy.
他克莫司（Tac）是一种有充分文献记载的免疫抑制剂，用于预防多种器官移植中的移植物抗宿主病。他克莫司狭窄的治疗窗和个体差异的药代动力学表明，作为其口服给药方案的一个重要概念，定期进行治疗药物监测（TDM）至关重要。通过去甲肾上腺素（NE）分子对银纳米颗粒（AgNPs）进行表面修饰，构建了一个简单、一步、选择性且灵敏的比色平台用于测定他克莫司。NE和Tac的附着会诱导AgNPs聚集，这可通过颜色区分（从黄色到棕色）以及可见光区域吸收峰的显著移动来观察到。所构建的纳米探针能够在2 ng/mL至70 ng/mL和70 ng/mL至1000 ng/mL两个线性范围内检测血浆样品中的他克莫司浓度，相关系数R>0.99。检测限（LOD）计算得出低至0.1 ng/mL。所开发的方法应用于他克莫司药物治疗下患者血浆样品中他克莫司的测定。
