Natural Product Research Center, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China; University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
Center for Traditional Chinese Medicine and Immunology Research School of Basic Medical Sciences, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, People's Republic of China; Department of Immunology and Pathogenic Biology, School of Basic Medical Sciences, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, People's Republic of China.
Bioorg Chem. 2022 Feb;119:105534. doi: 10.1016/j.bioorg.2021.105534. Epub 2021 Dec 2.
Fourteen previously undescribed diterpenoids, including an unusual diterpenoid (1) with a 9,10-seco-jatrophane skeleton, ten jatrophane-type diterpenoids (2-11), two lathyrane-type diterpenoids (12, 13), and an abietane-type diterpenoid (14), together with thirty-six known ones (15-50), were isolated from the whole plants of Euphorbia helioscopia L. The structures of the new isolates were characterized by spectroscopic methods, single-crystal X-ray diffraction analysis, and computational prediction of ECD and chemical shifts. Thirty-nine abundant diterpenoids were evaluated for their enhancement of NK cell-mediated killing of NSCLC cells. As a result, compounds 24, 33, and 41 were found to significantly enhance the killing activity of NK cells towards H1299-luci cells and A549-luci cells at the concentration of 2.5 μM.
从全株飞扬草中分离得到了 14 个新的二萜类化合物，包括一个具有 9,10-断节-jatrophane 骨架的不寻常二萜类化合物（1）、10 个 jatrophane 型二萜类化合物（2-11）、2 个 lathyrane 型二萜类化合物（12、13）和 1 个 abietane 型二萜类化合物（14），以及 36 个已知的化合物（15-50）。新分离物的结构通过光谱方法、单晶 X 射线衍射分析和 ECD 和化学位移的计算预测进行了表征。对 39 种丰富的二萜类化合物进行了评估，以考察它们对 NK 细胞介导的非小细胞肺癌细胞杀伤的增强作用。结果发现，在 2.5μM 的浓度下，化合物 24、33 和 41 显著增强了 NK 细胞对 H1299-luci 细胞和 A549-luci 细胞的杀伤活性。
