Institute of Lipid Metabolism and Atherosclerosis, Innovative Drug Research Centre, School of Pharmacy, Weifang Medical University, Baotongxi street 7166#, Weifang, Shandong province, China.
Lipids Health Dis. 2021 Dec 13;20(1):178. doi: 10.1186/s12944-021-01606-6.
Cordyceps militaris is cultured widely as an edible mushroom and accumulating evidence in mice have demonstrated that the polysaccharides of Cordyceps species have lipid-lowering effects. However, lipid metabolism in mice is significantly different from that in humans, making a full understanding of the mechanisms at play critical.
After 5 months, the hamsters were weighed and sampled under anesthesia after overnight fasting. The lipid-lowering effect and mechanisms of the polysaccharide CM1 was investigated by cellular and molecular technologies. Furthermore, the effect of the polysaccharide CM1 (100 μg/mL) on inhibiting adipocyte differentiation was investigated in vitro.
CM1, a polysaccharide from C. militaris, significantly decreased plasma total cholesterol, triglyceride and epididymal fat index in LDLR hamsters, which have a human-like lipid profile. After 5 months' administration, CM1 decreased the plasma level of apolipoprotein B48, modulated the expression of key genes and proteins in liver, small intestine, and epididymal fat. CM1 also inhibited preadipocyte differentiation in 3T3-L1 cells by downregulating the key genes involved in lipid droplet formation.
The polysaccharide CM1 lowers lipid and adipocyte differentiation by several pathways, and it has potential applications for hyperlipidemia prevention.
蛹虫草作为一种食用蘑菇被广泛培养，越来越多的证据表明，虫草属多糖具有降血脂作用。然而，小鼠的脂质代谢与人类有很大的不同，因此深入了解其作用机制至关重要。
经过 5 个月，在隔夜禁食后对麻醉状态下的仓鼠进行称重和采样。采用细胞和分子技术研究了多糖 CM1 的降脂作用及其机制。此外，还研究了多糖 CM1（100μg/ml）对体外脂肪细胞分化的抑制作用。
CM1 是一种来源于蛹虫草的多糖，可显著降低 LDLR 仓鼠的血浆总胆固醇、甘油三酯和附睾脂肪指数，使其具有类似人类的脂质谱。经过 5 个月的给药后，CM1 降低了血浆载脂蛋白 B48 的水平，调节了肝脏、小肠和附睾脂肪中关键基因和蛋白的表达。CM1 还通过下调参与脂滴形成的关键基因来抑制 3T3-L1 细胞前脂肪细胞的分化。
多糖 CM1 通过多种途径降低血脂和脂肪细胞分化，具有预防高血脂的应用潜力。
