Baccarini M, Kiderlen A F, Decker T, Lohmann-Matthes M L
Cell Immunol. 1986 Sep;101(2):339-50. doi: 10.1016/0008-8749(86)90147-4.
We previously reported that highly purified bone marrow-derived macrophage precursors can exert strong spontaneous cytotoxicity against YAC-1 tumor cells, Candida albicans, and protozoa of the genus Leishmania. In the present paper, evidence is shown that macrophage precursors in normal untreated mice are not confined to the bone marrow compartment but can also be found in the spleen. These organ-associated cells, which have the same buoyant density as large granular lymphocytes, have been positively sorted by means of an indirect rosetting technique employing the macrophage-specific monoclonal antibodies F4/80 and M143. The rosetting fractions represented an extremely homogeneous population of macrophage precursors characterized by high candidacidal and natural killer activity and by a strong proliferative response to the macrophage-specific colony-stimulating factor CSF-1. Spleen- and bone marrow-derived macrophage precursors differed in their target selectivity. In addition, the mature macrophages derived in vitro from these two precursor populations displayed striking differences in their candidacidal activity. The implications of these findings in relationship to heterogeneity in the macrophage differentiation line are discussed.
我们之前报道过，高度纯化的骨髓源性巨噬细胞前体能对YAC-1肿瘤细胞、白色念珠菌和利什曼原虫属的原生动物产生强烈的自发细胞毒性。在本文中，有证据表明，未经过处理的正常小鼠体内的巨噬细胞前体不仅局限于骨髓区室，在脾脏中也能找到。这些与器官相关的细胞，其浮力密度与大颗粒淋巴细胞相同，已通过采用巨噬细胞特异性单克隆抗体F4/80和M143的间接花环技术进行了阳性分选。花环组分代表了巨噬细胞前体的一个极其均一的群体，其特征是具有高杀念珠菌活性和自然杀伤活性，以及对巨噬细胞特异性集落刺激因子CSF-1有强烈的增殖反应。脾脏和骨髓来源的巨噬细胞前体在其靶标选择性上有所不同。此外，从这两个前体群体体外衍生的成熟巨噬细胞在其杀念珠菌活性方面表现出显著差异。本文讨论了这些发现与巨噬细胞分化谱系异质性的关系。
