Department of Materials and Environmental Chemistry, Stockholm University, 106 91 Stockholm, Sweden.
Drug Discov Today Technol. 2020 Dec;37:93-105. doi: 10.1016/j.ddtec.2020.12.002. Epub 2020 Dec 31.
Microcrystal electron diffraction (MicroED) has recently shown to be a promising technique for structure determination in structural biology and pharmaceutical chemistry. Here, we discuss the unique properties of electrons and motivate its use for diffraction experiments. We review the latest developments in MicroED, and illustrate its applications in macromolecular crystallography, fragment screening and structure guided drug discovery. We discuss the perspectives of MicroED in synthetic chemistry and pharmaceutical development. We anticipate that the rapid advances MicroED showcased here will promote further development of electron crystallography and open up new opportunities for drug discovery.
微晶电子衍射（MicroED）最近在结构生物学和药物化学领域的结构测定中显示出了很大的潜力。在这里，我们讨论了电子的独特性质，并说明了它在衍射实验中的应用。我们回顾了 MicroED 的最新进展，并举例说明了它在大分子晶体学、片段筛选和结构导向药物发现中的应用。我们还讨论了电子晶体学在合成化学和药物开发中的应用前景。我们预计，MicroED 的快速发展将推动电子晶体学的进一步发展，并为药物发现带来新的机遇。
