Ulshen M H, Lyn-Cook L E, Raasch R H
Gastroenterology. 1986 Nov;91(5):1134-40. doi: 10.1016/s0016-5085(86)80008-7.
To determine whether intraluminal administration of epidermal growth factor (EGF) has a trophic effect on small bowel mucosa, catheters were surgically placed in the ileum of adult rats and infused with EGF. Comparing animals receiving EGF (5 micrograms/48 h) with controls, in the ileum mean mucosal ornithine decarboxylase specific activity increased by greater than 200% (p less than 0.001), mean deoxyribonucleic acid specific activity and crypt labeling index increased by greater than or equal to 100% (p less than 0.001), and mean deoxyribonucleic acid content of the mucosa increased by 25% (p less than 0.05). During these studies, the jejunum was not exposed to ileal infusate, as shown with the use of a phenol red marker. Nevertheless, all measurements except deoxyribonucleic acid content increased in the jejunum as well, although to a lesser extent. A greater rise in mucosal ornithine decarboxylase and deoxyribonucleic acid specific activity could be demonstrated in the jejunum when EGF was infused directly into this segment. Mucosal ornithine decarboxylase activity was found to be dose-dependent and to increase in the ileum only after a latent period of 12-24 h. We conclude that intraluminal administration of EGF stimulates a mucosal proliferative response in the small intestine. Intraluminal EGF appears likely to be one of a number of endogenous trophic factors in the small bowel.
为了确定腔内给予表皮生长因子（EGF）对小肠黏膜是否具有营养作用，将导管手术置入成年大鼠的回肠并注入EGF。将接受EGF（5微克/48小时）的动物与对照组进行比较，回肠黏膜鸟氨酸脱羧酶的平均比活性增加超过200%（p<0.001），平均脱氧核糖核酸比活性和隐窝标记指数增加大于或等于100%（p<0.001），黏膜的平均脱氧核糖核酸含量增加25%（p<0.05）。在这些研究中，如使用酚红标记所示，空肠未接触回肠灌注液。然而，空肠中的所有测量值（除脱氧核糖核酸含量外）也都增加了，尽管增加幅度较小。当将EGF直接注入空肠段时，空肠黏膜鸟氨酸脱羧酶和脱氧核糖核酸比活性的升高更为明显。发现黏膜鸟氨酸脱羧酶活性具有剂量依赖性，且仅在12 - 24小时的潜伏期后回肠中的活性才增加。我们得出结论，腔内给予EGF可刺激小肠黏膜的增殖反应。腔内EGF似乎可能是小肠中多种内源性营养因子之一。
