Bamba T, Tsujikawa T, Hosoda S
Second Department of Internal Medicine, Shiga University of Medical Science, Otsu, Japan.
Gastroenterol Jpn. 1993 Aug;28(4):511-7. doi: 10.1007/BF02776949.
This study was undertaken to investigate the effect of epidermal growth factor (EGF) on the rat small intestinal mucosa by three different routes of administration. Four-week-old rats were fed elemental diet for 4 weeks and were administered EGF either subcutaneously, intraluminally or intraperitoneally with mini-osmotic pumps for a week. Intraperitoneal administration of EGF resulted in a significant increase of mucosal wet weight, mucosal content of protein and DNA, villus height, crypt depth and crypt cell production rate. Intraluminal or subcutaneous administration of EGF tended to increase those morphological and proliferative parameters, but did not cause any significant change. We conclude that EGF caused the hyperplasia of the small intestine of rats maintained on oral elemental diet and that this trophic effect was clearly shown by the intraperitoneal route of administration, rather than by the intraluminal route. These results suggest that EGF receptors located in the basal portion of crypt cells play a more important role than those located in the microvillous membrane.
本研究旨在通过三种不同给药途径探讨表皮生长因子（EGF）对大鼠小肠黏膜的影响。4周龄大鼠喂食要素饮食4周，然后用微型渗透泵皮下、肠腔内或腹腔内给予EGF一周。腹腔内给予EGF导致黏膜湿重、蛋白质和DNA的黏膜含量、绒毛高度、隐窝深度和隐窝细胞产生率显著增加。肠腔内或皮下给予EGF倾向于增加那些形态学和增殖参数，但未引起任何显著变化。我们得出结论，EGF导致口服要素饮食的大鼠小肠增生，并且这种营养作用通过腹腔给药途径而非肠腔内途径明显表现出来。这些结果表明，位于隐窝细胞基部的EGF受体比位于微绒毛膜上的受体发挥更重要的作用。
