Institute of Drug Metabolism and Pharmaceutical Analysis, Zhejiang Province Key Laboratory of Anti-Cancer Drug Research, College of Pharmaceutical Science, Zhejiang University, Hangzhou, Zhejiang, 310058, China.
First Affiliated Hospital of the Medical College, Shihezi University, Shihezi, Xinjiang, 832099, China.
Talanta. 2022 Mar 1;239:123129. doi: 10.1016/j.talanta.2021.123129. Epub 2021 Dec 7.
Mucin 1 (MUC1) overexpression in tumor cells is related to various cancers, including breast, stomach, and lung cancer. MUC1 detection and imaging are important for cancer localization in tissue sections to support histopathological diagnosis. In this study, we developed a simple, enzyme-free MUC1 detection and in situ imaging method. Three hairpin probes, Apt-trigger, HP1-FAM, and HP2, were designed for MUC1 recognition and hybridization chain reaction (HCR). The Apt-trigger probe was composed of two sequences: the MUC1 aptamer and HCR trigger sequence. The 5' end of the HP1-FAM probe was modified with a FAM signal molecule. In the presence of MUC1, the aptamer sequence is activated and bound to MUC1, which opens the hairpin structure. Then, the trigger sequence gets exposed and, complementary to HP1-FAM, triggers a continuous HCR process. This method was successfully used to detect MUC1 of 200 pM-25 nM and MUC1 in situ imaging in specific cells, such as human breast carcinoma (MCF-7) and human colon cancer (HT-29) cells.
粘蛋白 1（MUC1）在肿瘤细胞中的过度表达与多种癌症有关，包括乳腺癌、胃癌和肺癌。MUC1 的检测和成像对于组织切片中的癌症定位非常重要，有助于组织病理学诊断。在这项研究中，我们开发了一种简单、无酶的 MUC1 检测和原位成像方法。设计了三条发夹探针，Apt-trigger、HP1-FAM 和 HP2，用于 MUC1 的识别和杂交链式反应（HCR）。Apt-trigger 探针由两个序列组成：MUC1 适体和 HCR 触发序列。HP1-FAM 探针的 5'端修饰有 FAM 信号分子。在存在 MUC1 的情况下，适体序列被激活并与 MUC1 结合，从而打开发夹结构。然后，触发序列被暴露出来，并与 HP1-FAM 互补，触发连续的 HCR 过程。该方法成功地用于检测 200 pM-25 nM 的 MUC1 和特定细胞中的 MUC1 原位成像，例如人乳腺癌（MCF-7）和人结肠癌（HT-29）细胞。
