PEG-functionalized black phosphorus quantum dots as stable and biocompatible electrochemiluminescence luminophores for sensitive detection of tumor biomarker.

Despite black phosphorous (BP) QDs possess the merits of size-tunable band-gap, high electron mobility, and intrinsic defects, the spontaneous agglomeration and rapid oxidation of BP QDs in aqueous solution caused low electrochemiluminescence (ECL) efficiency and unstable ECL signal, which confined its further application of biological analysis. Herein, polyethylene glycol-functionalized BP QDs (PEG@BP QDs) were prepared showing an efficient and stable ECL response, which is attributed to the fact that PEG as protectant not only effectively prevented the spontaneous agglomeration, but also restrained the rapid oxidation of BP QDs in aqueous solution. As proof-of-concept, PEG@BP QDs were used as an efficient ECL emitter to combine with palindrome amplification-induced DNA walker to construct a sensitive ECL aptasensing platform for detecting cancer marker mucin 1 (MUC1). Interestingly, with the aid of positively charged thiolated PEG, the reaction rate of DNA walker on the electrode interface was clearly increased for the recovery of the ECL signal. The ECL aptasensor provides sensitive determination with the detection limit of 16.5 fg/mL. The proposed strategy paves a path for the development of efficient and stable ECL nanomaterials to construct biosensors for biosensing and clinical diagnosis.