Andrews B S, Schenk A, Barr R, Friou G, Mirick G, Ross P
J Am Acad Dermatol. 1986 Sep;15(3):474-81. doi: 10.1016/s0190-9622(86)70196-5.
Skin biopsy specimens obtained from involved skin from sixteen patients with systemic and discoid lupus erythematosus were studied. Murine monoclonal antibodies with a biotin-avidin-horseradish peroxidase staining system were used. The findings consisted of a marked reduction in the number of epidermal Langerhans cells defined by surface antigens, reduced HLA-DR (Ia-like) antigens on the surface of dermal capillary endothelium, and mononuclear cell infiltrates characterized by a predominance of helper T lymphocytes and an increase in the number of mononuclear phagocytic cells. B lymphocytes were rarely identified. The number of T lymphocytes within the dermis correlated inversely with both the number of HLA-DR-positive epidermal Langerhans cells (p less than 0.01) and the HLA-DR staining of dermal capillary endothelium (p less than 0.01). These findings suggest that a T lymphocyte-mediated immune response associated with a reduction in Langerhans cells and capillary endothelium HLA-DR antigens is involved in the inflammatory process of lupus erythematosus skin.
对16例系统性红斑狼疮和盘状红斑狼疮患者受累皮肤的活检标本进行了研究。采用了带有生物素-抗生物素蛋白-辣根过氧化物酶染色系统的鼠单克隆抗体。研究结果包括：由表面抗原定义的表皮朗格汉斯细胞数量显著减少；真皮毛细血管内皮表面的HLA-DR（类Ia）抗原减少；以辅助性T淋巴细胞为主的单核细胞浸润以及单核吞噬细胞数量增加。很少发现B淋巴细胞。真皮内T淋巴细胞的数量与HLA-DR阳性表皮朗格汉斯细胞的数量（p<0.01）以及真皮毛细血管内皮的HLA-DR染色（p<0.01）均呈负相关。这些发现提示，与朗格汉斯细胞和毛细血管内皮HLA-DR抗原减少相关的T淋巴细胞介导的免疫反应参与了红斑狼疮皮肤的炎症过程。
