Plant-Insect Interaction Group, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067, India.
Plant Biotic Interaction Lab, Department of Botany, University of Delhi, Delhi 110007, India.
Int J Mol Sci. 2023 Sep 12;24(18):13982. doi: 10.3390/ijms241813982.
BPH (brown planthopper) and WBPH (white backed planthopper) are significant rice pests that often co-occur as sympatric species and cause substantial yield loss. Despite their genetic similarities, different host-resistance genes confer resistance against these two hoppers. The defense mechanisms in rice against these pests are complex, and the molecular processes regulating their responses remain largely unknown. This study used specific recombinant inbred lines (RILs) derived from a cross between rice varieties RP2068-18-3-5 (BPH- and WBPH-resistant) and TN1 (BPH- and WBPH-susceptible) to investigate the mechanisms of interaction between these planthoppers and their rice hosts. WBPH and BPH were allowed to feed on specific RILs, and RNA-Seq was carried out on WBPH insects. Transcriptome profiling and qRT-PCR results revealed differential expression of genes involved in detoxification, digestion, transportation, cuticle formation, splicing, and RNA processing. A higher expression of sugar transporters was observed in both hoppers feeding on rice with resistance against either hopper. This is the first comparative analysis of gene expressions in these insects fed on genetically similar hosts but with differential resistance to BPH and WBPH. These results complement our earlier findings on the differential gene expression of the same RILs (BPH- or WBPH-infested) utilized in this study. Moreover, identifying insect genes and pathways responsible for countering host defense would augment our understanding of BPH and WBPH interaction with their rice hosts and enable us to develop lasting strategies to control these significant pests.
褐飞虱和白背飞虱是两种重要的水稻害虫，它们经常作为同域物种共同发生，并导致大量的产量损失。尽管它们具有遗传相似性，但不同的寄主抗性基因赋予了它们对这两种飞虱的抗性。水稻对这些害虫的防御机制很复杂，调节它们反应的分子过程在很大程度上仍然未知。本研究使用了特定的重组自交系（RILs），这些 RILs 是由水稻品种 RP2068-18-3-5（对褐飞虱和白背飞虱均具有抗性）和 TN1（对褐飞虱和白背飞虱均具有敏感性）杂交产生的，以研究这些飞虱与它们的水稻宿主之间的相互作用机制。允许白背飞虱和褐飞虱在特定的 RILs 上取食，并对白背飞虱进行 RNA-Seq 分析。转录组谱分析和 qRT-PCR 结果显示，参与解毒、消化、运输、表皮形成、剪接和 RNA 加工的基因表达存在差异。在两种飞虱取食对其中一种飞虱具有抗性的水稻时，都观察到了更高水平的糖转运蛋白的表达。这是首次对在遗传上相似的宿主上取食但对褐飞虱和白背飞虱具有不同抗性的这些昆虫的基因表达进行比较分析。这些结果补充了我们之前关于同一 RILs（褐飞虱或白背飞虱取食）的差异基因表达的研究结果。此外，确定昆虫基因和途径对宿主防御的反应，将增强我们对褐飞虱和白背飞虱与它们的水稻宿主相互作用的理解，并使我们能够制定持久的策略来控制这些重要的害虫。
