Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, China.
School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, China.
ACS Appl Mater Interfaces. 2021 Dec 22;13(50):59772-59786. doi: 10.1021/acsami.1c20565. Epub 2021 Dec 13.
Directed differentiation of bone marrow mesenchymal stem cells (BMSCs) toward chondrogenesis plays a predominant role in cartilage repair. However, the uncontrolled inflammatory response to implants is found to impair the stability of scaffolds and the cartilage regeneration outcome. Herein, we fabricated an injectable hydrogel crosslinked by strontium-doped bioglass (SrBG) to modulate both human BMSC (hBMSC) differentiation and the inflammatory response. The results revealed that the introduction of Sr ions could simultaneously enhance the proliferation of hBMSCs, upregulate cartilage-specific gene expression, and improve the secretion of glycosaminoglycan. Moreover, after cultured with SA/SrBG extracts in vitro, a majority of macrophages were polarized toward the M2 phenotype and subsequently facilitated the chondrogenic differentiation of hBMSCs. Furthermore, after the composite hydrogel was injected into a cartilage defect model, neonatal cartilage-like tissues with a smooth surface and tight integration with original tissues could be found. This study suggests that the synergistic strategy based on an enhanced differentiation ability and a regulated inflammatory response is promising and may lead the way to new anti-inflammatory biomaterials.
骨髓间充质干细胞（BMSCs）向软骨分化的定向分化在软骨修复中起着主要作用。然而，植入物的不受控制的炎症反应被发现会损害支架的稳定性和软骨再生的结果。在此，我们制备了一种由掺锶生物玻璃（SrBG）交联的可注射水凝胶，以调节人骨髓间充质干细胞（hBMSC）分化和炎症反应。结果表明，Sr 离子的引入可以同时增强 hBMSCs 的增殖，上调软骨特异性基因表达，并提高糖胺聚糖的分泌。此外，在体外与 SA/SrBG 提取物共培养后，大多数巨噬细胞向 M2 表型极化，并随后促进 hBMSCs 的软骨分化。此外，将复合水凝胶注入软骨缺损模型后，可发现表面光滑且与原组织紧密结合的新生儿软骨样组织。这项研究表明，基于增强的分化能力和调节的炎症反应的协同策略是有希望的，并可能为新的抗炎生物材料开辟道路。
