Perdoncini Gustavo, Sierra Arguello Yuli Melisa, Moreira Lima Leonardo, Quedi Furian Thales, Apellanis Borges Karen, Beatriz Rodrigues Laura, Ruschel Dos Santos Luciana, Borsoi Anderlise, Werlang Isolan Leonardo, Pereira Gomes Marcos José, Pippi Salle Carlos Tadeu, de Souza Moraes Hamilton Luiz, Pinheiro do Nascimento Vladimir
Departamento de Medicina Animal, Centro de Diagnóstico e Pesquisa em Patologia Aviária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Faculdade de Agronomia e Medicina Veterinária, Universidade de Passo Fundo, Passo Fundo, Brazil.
Foodborne Pathog Dis. 2022 Feb;19(2):143-150. doi: 10.1089/fpd.2021.0071. Epub 2021 Dec 13.
Campylobacteriosis is one of the most common bacteria causing human gastroenteritis. Poultry is a major reservoir of spp. as well as the main source of transmission. Due to the increased occurrence of campylobacteriosis, poultry slaughterhouses are under pressure to deliver carcasses with low contamination. However, a few studies have been carried out to evaluate contamination of broiler carcasses in Brazilian slaughter lines. Therefore, in this study, we aimed at detecting and quantifying the thermotolerant spp. at different stages of the poultry slaughtering process. The samples were collected from 12 points in three slaughterhouses in southern Brazil, at an interval of 12 months, and were tested for spp. by conventional microbiological technique, the most probable number, and real-time PCR. A total of 432 samples were analyzed. The majority of strains belonged to (92%), and the flock positivity among the three techniques was similar in most cases. was detected in all slaughtering stages. Although contamination has remained similar ( > 0.05) throughout almost all the slaughter process, evisceration seemed to be an important source of contamination. Our results reinforce the idea that the final carcass quality after the slaughtering process is directly influenced by the level of contamination of the broiler flocks on arrival at the processing plant.
弯曲杆菌病是引起人类肠胃炎的最常见细菌之一。家禽是弯曲杆菌属的主要宿主以及主要传播源。由于弯曲杆菌病发病率上升，家禽屠宰场面临着交付低污染胴体的压力。然而，巴西针对屠宰生产线中肉鸡胴体的污染情况进行评估的研究较少。因此，在本研究中，我们旨在检测和量化家禽屠宰过程不同阶段的耐热弯曲杆菌属。样本从巴西南部三个屠宰场的12个点采集，间隔12个月，并通过传统微生物技术、最大可能数法和实时荧光定量PCR检测弯曲杆菌属。共分析了432个样本。大多数菌株属于空肠弯曲杆菌（92%），在大多数情况下，三种技术检测的鸡群阳性率相似。在所有屠宰阶段均检测到弯曲杆菌。尽管在几乎整个屠宰过程中污染情况保持相似（P＞0.05），但去内脏似乎是一个重要的污染源。我们的结果强化了这样一种观点，即屠宰过程后的最终胴体质量直接受到肉鸡群抵达加工厂时的污染水平的影响。
