Department of Obstetrics and Gynaecology, The Second Clinical College, Guizhou University of Chinese Medicine, Guiyang, China.
Reproductive Centre,Women and Children's Hospital, Qingdao University, Qingdao, China.
Bioengineered. 2021 Dec;12(2):10470-10479. doi: 10.1080/21655979.2021.1997367.
Controlled ovarian hyperstimulation (COH) impairs the endometrium receptivity during the implantation window, resulting in a lower clinical pregnancy rate and a higher abortion rate. Our study explored the effect of electroacupuncture on the endometrial receptivity of COH rats. Female rats were randomly divided into normal treatment (Normal), model treatment (Model), low-frequency electroacupuncture treatment (LF-EA) and high-frequency electroacupuncture treatment (HF-EA). Rats in the Model, LF-EA, and HF-EA treatment groups were injected with pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (HCG) to establish a model of COH rats. Compared with the Normal, the endometrial thickness, the number of pinopodes and amount of blastocyst implantation in the Model group were significantly reduced. Among them, the endometrial thickness and the amount of blastocyst implantation in the Model group were substantially decreased than those in the HF-EA group. High-frequency electroacupuncture treatment could markedly reduce the protein expression levels of E-cadherin, β-catenin and claudin-1 (CLDN1). During HF-EA treatment, the LIF/STAT3 signaling pathway of COH rats was enhanced. In conclusion, electroacupuncture could improve the endometrium receptivity and promote the blastocyst implantation in COH rats by reducing cell adhesion molecules and enhancing the LIF/STAT3 signaling pathway. High-frequency electroacupuncture could effectively improve endometrial receptivity and blastocyst implantation amount in COH rats.Electroacupuncture, especially high-frequency electroacupuncture, could significantly increase endometrial thickness and the number of pinopodes.High-frequency electroacupuncture significantly reduced the protein expression levels of E-cadherin, β-catenin and CLDN1 adhesion molecules in COH rats.High-frequency electroacupuncture could markedly enhance the LIF/STAT3 signaling pathway in COH rats.
控制性卵巢超刺激（COH）会损害着床窗口期子宫内膜的容受性，导致临床妊娠率降低和流产率升高。本研究探讨了电针对 COH 大鼠子宫内膜容受性的影响。雌性大鼠随机分为正常治疗组（Normal）、模型治疗组（Model）、低频电针治疗组（LF-EA）和高频电针治疗组（HF-EA）。Model、LF-EA 和 HF-EA 治疗组大鼠注射孕马血清促性腺激素（PMSG）和人绒毛膜促性腺激素（HCG）建立 COH 大鼠模型。与 Normal 组相比，Model 组的子宫内膜厚度、微绒毛数量和胚胎囊着床数量明显减少。其中，Model 组的子宫内膜厚度和胚胎囊着床数量明显低于 HF-EA 组。高频电针治疗可显著降低 E-钙黏蛋白、β-连环蛋白和紧密连接蛋白 1（CLDN1）的蛋白表达水平。在 HF-EA 治疗过程中，COH 大鼠的 LIF/STAT3 信号通路被增强。总之，电针通过降低细胞黏附分子和增强 LIF/STAT3 信号通路，可改善 COH 大鼠的子宫内膜容受性，促进胚胎囊着床。高频电针可有效改善 COH 大鼠的子宫内膜容受性和胚胎囊着床数量。电针，尤其是高频电针，可显著增加 COH 大鼠的子宫内膜厚度和微绒毛数量。高频电针可显著降低 COH 大鼠细胞黏附分子 E-钙黏蛋白、β-连环蛋白和 CLDN1 的蛋白表达水平。高频电针可显著增强 COH 大鼠的 LIF/STAT3 信号通路。
