Jiang Chunqi, Oshin Edwin A, Guo Siqi, Scott Megan, Li Xi, Mangiamele Cathryn, Heller Richard
Frank Reidy Research Center for Bioelectrics and the Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, VA 23529 USA.
Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23529 USA.
IEEE Trans Plasma Sci IEEE Nucl Plasma Sci Soc. 2021 Nov;49(11):3317-3324. doi: 10.1109/tps.2021.3113260. Epub 2021 Sep 30.
Nonthermal atmospheric pressure plasmas produce reactive plasma species including charged particles and reactive oxygen nitrogen species, which are known to induce oxidative stress in living cells in liquid or tissue. In the meantime, pulsed electric fields have been widely used in reversible or irreversible electropermeabilization for either the delivery of plasmid DNA or inactivation of cancer cells. This work discusses the synergistic effects of nanosecond pulsed plasma jets and pulsed electric field on inactivation of pancreatic cancer cells and enhancement of plasmid DNA delivery to guinea pig skin . Higher inactivation rates of the cancer cells in suspension were obtained with combined treatment of 300-ns 50 kV/cm pulsed electric field and a 1-min exposure of a nanosecond pulsed, 250-m plasma jet. Increased efficiency of gene electrotransfer to skin was also observed after a 3-min treatment of a nanosecond pulsed, 1-mm plasma jet. Application of the plasma alone at the same dosage did not have significant effect on gene delivery. These findings signify the dosage-dependent cell-response to both the electric fields and plasma. Importantly, the use of cold plasma to increase the sensitization of the biological cells in response to pulsed electric fields could be an effective approach to enhance the desired effects in electroporation-based applications.
非热大气压等离子体产生包括带电粒子和活性氧氮物种在内的活性等离子体物质，已知这些物质会在液体或组织中的活细胞中诱导氧化应激。与此同时，脉冲电场已广泛用于可逆或不可逆的电穿孔，用于质粒DNA的递送或癌细胞的灭活。这项工作讨论了纳秒脉冲等离子体射流和脉冲电场对胰腺癌细胞灭活以及增强质粒DNA递送至豚鼠皮肤的协同作用。通过300纳秒50 kV/cm脉冲电场与纳秒脉冲250米等离子体射流1分钟暴露的联合处理，获得了悬浮癌细胞更高的失活率。在对1毫米纳秒脉冲等离子体射流进行3分钟处理后，还观察到基因电转移至皮肤的效率提高。以相同剂量单独应用等离子体对基因递送没有显著影响。这些发现表明细胞对电场和等离子体的反应存在剂量依赖性。重要的是，使用冷等离子体来增加生物细胞对脉冲电场的敏感性可能是一种有效的方法，可以在基于电穿孔的应用中增强所需的效果。
