Univ. Limoges, CNRS, XLIM, UMR 7252, 87000, Limoges, France.
SIAME Laboratory, University of Pau, 64013, Pau, France.
Med Biol Eng Comput. 2018 Jan;56(1):85-97. doi: 10.1007/s11517-017-1676-0. Epub 2017 Jul 4.
In this paper, delivery devices for nanosecond pulsed electric field exposure of biological samples in direct contact with electrodes or isolated are presented and characterized. They are based on a modified electroporation cuvette and two transverse electromagnetic cells (TEM cells). The devices were used to apply pulses with high intensity (4.5 kV) and short durations (3 and 13 ns). The delivery devices were electromagnetically characterized in the frequency and time domains. Field intensities of around 5, 0.5, and 12 MV m were obtained by numerical simulations of the biological sample positioned in the three delivery devices. Two delivery systems had a homogenous electric field spatial distribution, and one was adapted to permit a highly localized exposure in the vicinity of a needle. Experimental biological investigations were carried out at different field intensities for five cancer cell lines. The results using flow cytometry showed that cells kept polarized mitochondrial membrane but lost plasma membrane integrity following a dose-response trend after exposure to different electric field intensities. Certain cell types (U87, MCF7) showed higher sensitivities to nsPEFs than other lines tested.
本文介绍并表征了用于在直接与电极接触或隔离的情况下对生物样品进行纳秒脉冲电场暴露的输送装置。它们基于改进的电穿孔比色皿和两个横向电磁细胞（TEM 细胞）。这些装置用于施加高强度（4.5 kV）和短持续时间（3 和 13 ns）的脉冲。通过对放置在三个输送装置中的生物样品进行数值模拟，对输送装置进行了频域和时域的电磁特性表征。在两种输送系统中，获得了约 5、0.5 和 12 MV/m 的场强，其中一个输送系统的空间电场分布均匀，另一个系统则适用于在针附近进行高度局部化的暴露。在不同场强下对五种癌细胞系进行了实验生物学研究。使用流式细胞术的结果表明，细胞保持极化的线粒体膜，但在暴露于不同场强后，根据剂量反应趋势失去了质膜的完整性。某些细胞类型（U87、MCF7）对 nsPEF 的敏感性高于其他测试的细胞系。
