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骨膜蛋白对成骨细胞在胶原基质上黏附与增殖的影响——一项研究。

The influence of periostin on osteoblastic adhesion and proliferation on collagen matrices - An study.

作者信息

Mohanarangam Suganthi, Victor Dhayanand John, Subramanian Sangeetha, Prakash Psg

机构信息

Consultant Periodontist, Thiruvannamalai, Faculty, Chennai, Tamil Nadu, India.

Department of Periodontics, SRM Dental College and Hospital, Chennai, Tamil Nadu, India.

出版信息

J Indian Soc Periodontol. 2021 Nov-Dec;25(6):480-484. doi: 10.4103/jisp.jisp_396_20. Epub 2021 Nov 1.

DOI:10.4103/jisp.jisp_396_20
PMID:34898912
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8603795/
Abstract

PURPOSE

The purpose of the study was to evaluate the ability of periostin when impregnated onto varied collagen matrices to influence osteoblast cell adhesion, proliferation, and activity.

MATERIALS AND METHODS

Saos-2 osteoblast cells were cultured and seeded onto two different collagen matrices as follows: Group A: absorbable collagen sponge (ACS), Group B: ACS impregnated with recombinant human periostin, Group C: nanocrystalline hydroxyapatite collagen (NcHC), and Group D: NcHC impregnated with recombinanant human periostin. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed to evaluate cell viability as well as adhesion and proliferation on 2, 5, and 7 day. Osteoblast activity was studied using alkaline phosphatase (ALP) assay for the study groups.

RESULTS

The periostin-treated absorbable collagen matrices showed a statistically significant increase in the osteoblast adhesion compared to periostin-treated NcHC on days 2, 5, and 7 ( < 0.001). The osteoblast activity as evaluated by ALP assay showed that there is increased activity in the periostin-treated ACS compared to the periostin-treated NcHC.

CONCLUSION

From the observations of this study, it is evident that Periostin has a significant role in the modulating cellular response of the osteoblast cells. Further, incorporation of periostin into the ACS has been shown to increase the cell viability, proliferation, and adhesion of osteoblast-like Saos-2 cells.

摘要

目的

本研究的目的是评估骨膜蛋白负载于不同胶原蛋白基质时对成骨细胞黏附、增殖及活性的影响能力。

材料与方法

培养Saos-2成骨细胞并接种于两种不同的胶原蛋白基质上，如下：A组：可吸收胶原蛋白海绵（ACS）；B组：负载重组人骨膜蛋白的ACS；C组：纳米晶羟基磷灰石胶原蛋白（NcHC）；D组：负载重组人骨膜蛋白的NcHC。采用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐法在第2、5和7天评估细胞活力以及黏附与增殖情况。使用碱性磷酸酶（ALP）检测法对各研究组的成骨细胞活性进行研究。

结果

在第2、5和7天，与负载骨膜蛋白的NcHC相比，负载骨膜蛋白的可吸收胶原蛋白基质上的成骨细胞黏附具有统计学显著增加（<0.001）。通过ALP检测法评估的成骨细胞活性显示，与负载骨膜蛋白 的NcHC相比，负载骨膜蛋白的ACS中的活性有所增加。

结论

从本研究的观察结果来看，显然骨膜蛋白在调节成骨细胞的细胞反应中具有重要作用。此外，已证明将骨膜蛋白掺入ACS可提高成骨样Saos-2细胞的细胞活力、增殖及黏附。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3650/8603795/ca5659cfc43a/JISP-25-480-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3650/8603795/035da3b3bb18/JISP-25-480-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3650/8603795/b1efa3b0313d/JISP-25-480-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3650/8603795/ab8c44025b8a/JISP-25-480-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3650/8603795/f042fa9b816c/JISP-25-480-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3650/8603795/ca5659cfc43a/JISP-25-480-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3650/8603795/035da3b3bb18/JISP-25-480-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3650/8603795/b1efa3b0313d/JISP-25-480-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3650/8603795/ab8c44025b8a/JISP-25-480-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3650/8603795/f042fa9b816c/JISP-25-480-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3650/8603795/ca5659cfc43a/JISP-25-480-g005.jpg

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The multiple facets of periostin in bone metabolism.
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