Catrileo Daniela, Moreira Sandra, Ganga María Angélica, Godoy Liliana
Departamento de Fruticultura y Enología, Facultad de Agronomía e Ingeniería Forestal, Pontificia Universidad Católica de Chile, Santiago, Chile.
Departamento de Ciencia y Tecnología de los Alimentos, Facultad Tecnológica, Universidad de Santiago de Chile, Santiago, Chile.
Front Microbiol. 2021 Nov 25;12:747868. doi: 10.3389/fmicb.2021.747868. eCollection 2021.
is considered the most significant contaminant yeast in the wine industry since it causes a deterioration in the organoleptic properties of the wine and significant economic losses. This deterioration is due to the production of volatile phenols from hydroxycinnamic acids. These compounds possess antimicrobial properties; however, can resist this effect because it metabolizes them into less toxic ones. Recent studies have reported that grows under different stress conditions, including -coumaric acid (CA) but effective methods for its control have not been found yet. Since that in other yeasts, such as , it has been described that light affects its growth, and we evaluated whether the light would have a similar effect on . The results show that at light intensities of 2,500 and 4,000 lux in the absence of CA, LAMAP2480 does not grow in the culture medium; however, when the medium contains this acid, the yeast adapts to both factors of stress managing to grow. The expression of genes related to oxidative stress in LAMAP2480, such as , , and , showed a higher relative expression when the yeast was exposed to 2,500 lux compared to 4,000 lux, agreeing with the growth curves. This suggests that a higher expression of the genes studied would be related to stress-protective effects by CA.
被认为是葡萄酒行业中最主要的污染酵母，因为它会导致葡萄酒感官特性变差并造成重大经济损失。这种变质是由于从羟基肉桂酸产生挥发性酚类物质所致。这些化合物具有抗菌特性；然而，[具体酵母名称]能够抵抗这种影响，因为它将它们代谢为毒性较小的物质。最近的研究报告称，[具体酵母名称]在不同的应激条件下生长，包括对香豆酸（CA），但尚未找到有效的控制方法。由于在其他酵母中，如[其他酵母名称]，已经描述光照会影响其生长，我们评估了光照对[具体酵母名称]是否有类似影响。结果表明，在没有CA的情况下，当光照强度为2500勒克斯和4000勒克斯时，[具体酵母名称]LAMAP2480在培养基中不生长；然而，当培养基含有这种酸时，酵母能够适应这两种应激因素并设法生长。与生长曲线一致，当酵母暴露于2500勒克斯而不是4000勒克斯时，[具体酵母名称]LAMAP2480中与氧化应激相关的基因，如[基因名称1]、[基因名称2]和[基因名称3]的表达显示出更高的相对表达。这表明所研究基因的较高表达可能与CA的应激保护作用有关。
