Zheng Lei, Wang Xue, Ding Aizhong, Yuan Dongdan, Tan Qiuyang, Xing Yuzi, Xie En
College of Water Sciences, Beijing Normal University, Beijing, China.
College of Water Resources and Civil Engineering, China Agricultural University, Beijing, China.
Front Microbiol. 2021 Nov 10;12:779369. doi: 10.3389/fmicb.2021.779369. eCollection 2021.
The microbial characteristics in the wastewater treatment plants (WWTPs) strongly affect their optimal performance and functional stability. However, a cognitive gap remains regarding the characteristics of the microbial community driven by phosphorus sources, especially co-occurrence patterns and community assembly based on phylogenetic group. In this study, 59 denitrifying phosphorus removal (DPR) activated sludge samples were cultivated with phosphorus sources. The results suggested that homogeneous selection accounted for the largest proportion that ranged from 35.82 to 64.48%. Deterministic processes dominated in 12 microbial groups (bins): Accumulibacter and in these bins belonged to phosphate-accumulating organisms (PAOs). Network analysis revealed that species interactions were intensive in cyclic nucleoside phosphate-influenced microbiota. Function prediction indicated that cyclic nucleoside phosphates increased the activity of enzymes related to denitrification and phosphorus metabolism and increased the α-diversity of microorganism but decreased the diversity of metabolic function. Based on these results, it was assumed that cyclic nucleoside phosphates, rather than inorganic phosphates, are the most available phosphorus source for majority microorganisms in DPR activated sludge. The study revealed the important role of phosphorus source in the construction and assembly of microbial communities and provided new insights about pollutant removal from WWTPs.
污水处理厂（WWTPs）中的微生物特性强烈影响其最佳性能和功能稳定性。然而，对于由磷源驱动的微生物群落特征，尤其是基于系统发育组的共现模式和群落组装，仍存在认知差距。在本研究中，用磷源培养了59个反硝化除磷（DPR）活性污泥样本。结果表明，均匀选择占比最大，范围为35.82%至64.48%。确定性过程在12个微生物组（分类单元）中占主导：聚磷菌属（Accumulibacter），这些分类单元中的微生物属于聚磷菌（PAOs）。网络分析表明，在环核苷酸影响的微生物群中物种相互作用强烈。功能预测表明，环核苷酸增加了与反硝化和磷代谢相关的酶的活性，增加了微生物的α多样性，但降低了代谢功能的多样性。基于这些结果，推测环核苷酸而非无机磷酸盐是DPR活性污泥中大多数微生物最易利用的磷源。该研究揭示了磷源在微生物群落构建和组装中的重要作用，并为污水处理厂污染物去除提供了新见解。
