Wang Chun, Yuan Zixi, Sun Yingxue, Yao Xiaolong, Li Ruixuan, Li Shuangshuang
School of Ecology and Environment, Beijing Technology and Business University, Beijing, China.
State Environmental Protection Key Laboratory of Food Chain Pollution Control, Beijing Technology and Business University, Beijing, China.
Front Microbiol. 2021 Nov 25;12:782611. doi: 10.3389/fmicb.2021.782611. eCollection 2021.
The ever-increasing production and processing of textiles will lead to greater risks of releasing pollutants into the environment. Textile wastewater treatment plants (TWTPs) effluent are an important source of persistent toxic pollutants in receiving water bodies. The effects of specific pollutants on organisms are usually studied under laboratory conditions, and therefore, comprehensive results are not obtained regarding the chronic combined effects of pollutants under aquatic environmental conditions. Thus, this study aimed to determine the combined effects of TWTP effluents on the growth performance, oxidative stress, inflammatory response, and intestinal microbiota of adult zebrafish (). Exposure to TWTP effluents significantly inhibited growth, exacerbated the condition factor, and increased the mortality of adult zebrafish. Moreover, markedly decreases were observed in the activities of antioxidant enzymes, such as CAT, GSH, GSH-Px, MDA, SOD, and T-AOC, mostly in the intestine and muscle tissues of zebrafish after 1 and 4 months of exposure. In addition, the results demonstrated that TWTP effluent exposure affected the intestinal microbial community composition and decreased community diversity. Slight changes were found in the relative abundance of probiotic , , and in zebrafish guts after chronic TWTP effluent exposure. The chronic toxic effects of slight increases in opportunistic pathogens, such as , , and , deserve further attention. Our results reveal that TWTP effluent exposure poses potential health risks to aquatic organisms through growth inhibition, oxidative stress impairment of the intestine and muscles, and intestinal microbial community alterations.
纺织品生产和加工的不断增加将导致向环境中释放污染物的风险更大。纺织废水处理厂（TWTPs）的废水是受纳水体中持久性有毒污染物的重要来源。特定污染物对生物体的影响通常在实验室条件下进行研究，因此，未获得关于水生环境条件下污染物慢性联合效应的全面结果。因此，本研究旨在确定纺织废水处理厂废水对成年斑马鱼生长性能、氧化应激、炎症反应和肠道微生物群的联合影响。暴露于纺织废水处理厂废水显著抑制了成年斑马鱼的生长，加剧了肥满度，并增加了死亡率。此外，在暴露1个月和4个月后，主要在斑马鱼的肠道和肌肉组织中观察到抗氧化酶如CAT、GSH、GSH-Px、MDA、SOD和T-AOC的活性显著降低。此外，结果表明，暴露于纺织废水处理厂废水会影响肠道微生物群落组成并降低群落多样性。长期暴露于纺织废水处理厂废水后，斑马鱼肠道中益生菌、和的相对丰度发生了轻微变化。机会性病原体如、和的轻微增加所产生的慢性毒性作用值得进一步关注。我们的结果表明，暴露于纺织废水处理厂废水会通过生长抑制、肠道和肌肉的氧化应激损伤以及肠道微生物群落改变对水生生物构成潜在健康风险。
