Li Shuangshuang, Cai Mingcan, Wang Qing, Yuan Zixi, Li Ruixuan, Wang Chun, Sun Yingxue
College of Energy and Environmental Engineering, Hebei University of Engineering, Handan, 056038, China.
Key Laboratory of Environment Controlled Aquaculture (Dalian Ocean University) Ministry of Education, Dalian, 116023, China.
Environ Sci Pollut Res Int. 2023 Apr;30(18):53674-53684. doi: 10.1007/s11356-023-26167-2. Epub 2023 Mar 3.
Strict standards have been put forward for the treatment and discharge of dyeing wastewater worldwide. However, there are still traces amount of pollutants, especially emerging pollutants in dyeing wastewater treatment plant (DWTP) effluent. Few studies have focused on the chronic biological toxicity effect and mechanism of DWTP effluent. In this study, 3-month chronic compound toxic effects were investigated by the exposure of DWTP effluent using adult zebrafish. Significantly higher mortality and fatness and significantly lower body weight and body length were found in the treatment group. In addition, long-term exposure to DWTP effluent also obviously reduced liver-body weight ratio of zebrafish, causing abnormal liver development of zebrafish. Moreover, DWTP effluent led to obvious changes in the gut microbiota and microbial diversity of zebrafish. At phylum level, significantly higher of Verrucomicrobia but lower Tenericutes, Actinobacteria, and Chloroflexi were found in the control group. At genus level, the treatment group had significantly higher abundance of Lactobacillus, but significantly lower abundance of Akkermansia, Prevotella, Bacteroides, and Sutterella. These results suggested that long-term exposure to DWTP effluent led to imbalance of gut microbiota in zebrafish. In general, this research indicated that DWTP effluent pollutants could result in negative health outcomes to aquatic organisms.
全球已针对印染废水的处理和排放提出了严格标准。然而，印染废水处理厂（DWTP）的出水仍含有痕量污染物，尤其是新兴污染物。很少有研究关注DWTP出水的慢性生物毒性效应及其机制。在本研究中，通过将成年斑马鱼暴露于DWTP出水中，研究了为期3个月的慢性复合毒性效应。结果发现，处理组的死亡率和肥满度显著更高，而体重和体长显著更低。此外，长期暴露于DWTP出水还明显降低了斑马鱼的肝体比，导致斑马鱼肝脏发育异常。此外，DWTP出水导致斑马鱼肠道微生物群和微生物多样性发生明显变化。在门水平上，对照组中疣微菌门的比例显著更高，而柔膜菌门、放线菌门和绿弯菌门的比例更低。在属水平上，处理组中乳酸杆菌的丰度显著更高，但阿克曼氏菌、普雷沃氏菌、拟杆菌和萨特氏菌的丰度显著更低。这些结果表明，长期暴露于DWTP出水会导致斑马鱼肠道微生物群失衡。总体而言，本研究表明DWTP出水污染物会对水生生物的健康产生负面影响。
