Li Candong, Jiang Hongwei, Li Yingying, Liu Chunyan, Qi Zhaoming, Wu Xiaoxia, Zhang Zhanguo, Hu Zhenbang, Zhu Rongsheng, Guo Tai, Wang Zhixin, Zheng Wei, Zhang Zhenyu, Zhao Haihong, Wang Nannan, Shan Dapeng, Xin Dawei, Luan Feishi, Chen Qingshan
Jiamusi Branch Institute, Heilongjiang Academy of Agricultural Sciences, Jiamusi, China.
College of Life Science, Northeast Agricultural University, Harbin, China.
Front Plant Sci. 2021 Nov 26;12:715488. doi: 10.3389/fpls.2021.715488. eCollection 2021.
The three-seeded pod number is an important trait that positively influences soybean yield. Soybean variety with increased three-seeded pod number contributes to the seed number/plant and higher yield. The candidate genes of the three-seeded pod may be the key for improving soybean yield. In this study, identification and validation of candidate genes for three-seeded pod has been carried out. First, a total of 36 quantitative trait locus (QTL) were detected from the investigation of recombinant inbred lines including 147 individuals derived from a cross between Charleston and Dongning 594 cultivars. Five consensus QTLs were integrated. Second, an introgressed line CSSL-182 carrying the target segment for the trait from the donor parent was selected to verify the consensus QTL based on its phenotype. Third, a secondary group was constructed by backcrossing with CSSL-182, and two QTLs were confirmed. There were a total of 162 genes in the two QTLs. The mining of candidate genes resulted in the annotation of eight genes with functions related to pod and seed sets. Finally, haplotype analysis and quantitative reverse transcriptase real-time PCR were carried to verify the candidate genes. Four of these genes had different haplotypes in the resource group, and the differences in the phenotype were highly significant. Moreover, the differences in the expression of the four genes during pod and seed development were also significant. These four genes were probably related to the development process underlying the three-seeded pod in soybean. Herein, we discuss the past and present studies related to the three-seeded pod trait in soybean.
三粒荚数量是对大豆产量有积极影响的重要性状。具有增加的三粒荚数量的大豆品种有助于提高单株粒数和产量。三粒荚的候选基因可能是提高大豆产量的关键。在本研究中，已对三粒荚的候选基因进行了鉴定和验证。首先，通过对包括147个个体的重组自交系进行调查，这些个体源自Charleston和东宁594品种的杂交，共检测到36个数量性状位点（QTL）。整合了5个共有QTL。其次，选择携带来自供体亲本的该性状目标片段的导入系CSSL-182，根据其表型验证共有QTL。第三，通过与CSSL-182回交构建次级群体，确认了2个QTL。这2个QTL中共有162个基因。对候选基因的挖掘导致注释了8个与荚果和种子形成相关功能的基因。最后，进行单倍型分析和定量逆转录实时PCR以验证候选基因。这些基因中的4个在资源群体中有不同的单倍型，表型差异极显著。此外，这4个基因在荚果和种子发育过程中的表达差异也很显著。这四个基因可能与大豆三粒荚的发育过程有关。在此，我们讨论了与大豆三粒荚性状相关的过去和现在的研究。
