Jiang Hongwei, Li Yingying, Qin Hongtao, Li Yongliang, Qi Huidong, Li Candong, Wang Nannan, Li Ruichao, Zhao Yuanyuan, Huang Shiyu, Yu Jingyao, Wang Xinyu, Zhu Rongsheng, Liu Chunyan, Hu Zhenbang, Qi Zhaoming, Xin Dawei, Wu Xiaoxia, Chen Qingshan
College of Agriculture, Northeast Agricultural University, Harbin, China.
Jilin Academy of Agricultural Sciences, Soybean Research Institute, Changchun, China.
Front Plant Sci. 2018 Sep 19;9:1280. doi: 10.3389/fpls.2018.01280. eCollection 2018.
First pod height (FPH) is a quantitative trait in soybean [ (L.) Merr.] that affects mechanized harvesting. A compatible combination of the FPH and the mechanized harvester is required to ensure that the soybean is efficiently harvested. In this study, 147 recombinant inbred lines, which were derived from a cross between 'Dongnong594' and 'Charleston' over 8 years, were used to identify the major quantitative trait loci (QTLs) associated with FPH. Using a composite interval mapping method with WinQTLCart (version 2.5), 11 major QTLs were identified. They were distributed on five soybean chromosomes, and 90 pairs of QTLs showed significant epistatic associates with FPH. Of these, 3 were main QTL × main QTL interactions, and 12 were main QTL × non-main QTL interactions. A KEGG gene annotation of the 11 major QTL intervals revealed 8 candidate genes related to plant growth, appearing in the pathways K14486 (auxin response factor 9), K14498 (serine/threonine-protein kinase), and K13946 (transmembrane amino acid transporter family protein), and 7 candidate genes had high expression levels in the soybean stems. These results will aid in building a foundation for the fine mapping of the QTLs related to FPH and marker-assisted selection for breeding in soybean.
第一节荚高度（FPH）是大豆[（L.）Merr.]中的一个数量性状，它影响机械化收获。需要FPH与机械化收割机的兼容组合，以确保大豆能够高效收获。在本研究中，使用了147个重组自交系，这些自交系是由‘东农594’和‘查尔斯顿’杂交8年衍生而来的，用于鉴定与FPH相关的主要数量性状位点（QTL）。使用WinQTLCart（版本2.5）的复合区间作图方法，鉴定出11个主要QTL。它们分布在五条大豆染色体上，90对QTL与FPH表现出显著的上位性关联。其中，3对是主QTL×主QTL互作，12对是主QTL×非主QTL互作。对11个主要QTL区间进行KEGG基因注释，发现8个与植物生长相关的候选基因，出现在K14486（生长素响应因子9）、K14498（丝氨酸/苏氨酸蛋白激酶）和K13946（跨膜氨基酸转运蛋白家族蛋白）途径中，7个候选基因在大豆茎中具有高表达水平。这些结果将有助于为大豆中与FPH相关的QTL精细定位和标记辅助选择育种奠定基础。
