Dhakal Smit, Liu Xiaoxiao, Chu Chenggen, Yang Yan, Rudd Jackie C, Ibrahim Amir M H, Xue Qingwu, Devkota Ravindra N, Baker Jason A, Baker Shannon A, Simoneaux Bryan E, Opena Geraldine B, Sutton Russell, Jessup Kirk E, Hui Kele, Wang Shichen, Johnson Charles D, Metz Richard P, Liu Shuyu
Texas A&M AgriLife Research and Extension Center, Texas A&M AgriLife Research, Amarillo, TX, United States of America.
Edward T. Schafer Agricultural Research Center, Sugarbeet & Potato Research Unit, USDA-ARS, Fargo, ND, United States of America.
PeerJ. 2021 Nov 24;9:e12350. doi: 10.7717/peerj.12350. eCollection 2021.
Quantitative trait loci (QTL) analysis could help to identify suitable molecular markers for marker-assisted breeding (MAB). A mapping population of 124 Frecombinant inbred lines derived from the cross 'TAM 112'/'TAM 111' was grown under 28 diverse environments and evaluated for grain yield, test weight, heading date, and plant height. The objective of this study was to detect QTL conferring grain yield and agronomic traits from multiple mega-environments. Through a linkage map with 5,948 single nucleotide polymorphisms (SNPs), 51 QTL were consistently identified in two or more environments or analyses. Ten QTL linked to two or more traits were also identified on chromosomes 1A, 1D, 4B, 4D, 6A, 7B, and 7D. Those QTL explained up to 13.3% of additive phenotypic variations with the additive logarithm of odds (LOD(A)) scores up to 11.2. The additive effect increased yield up to 8.16 and 6.57 g m and increased test weight by 2.14 and 3.47 kg m with favorable alleles from TAM 111 and TAM 112, respectively. Seven major QTL for yield and six for TW with one in common were of our interest on MAB as they explained 5% or more phenotypic variations through additive effects. This study confirmed previously identified loci and identified new QTL and the favorable alleles for improving grain yield and agronomic traits.
数量性状位点（QTL）分析有助于识别适合用于标记辅助育种（MAB）的分子标记。由杂交组合“TAM 112”/“TAM 111”衍生出的124个重组自交系的作图群体，在28种不同环境下种植，并对籽粒产量、容重、抽穗期和株高进行了评估。本研究的目的是从多个大环境中检测赋予籽粒产量和农艺性状的QTL。通过一张包含5948个单核苷酸多态性（SNP）的连锁图谱，在两个或更多环境或分析中一致鉴定出51个QTL。在1A、1D、4B、4D、6A、7B和7D染色体上还鉴定出10个与两个或更多性状相关的QTL。这些QTL解释了高达13.3%的加性表型变异，加性对数优势（LOD(A)）分数高达11.2。来自TAM 111和TAM 112的有利等位基因分别使产量增加了8.16和6.57 g/m²，容重增加了2.14和3.47 kg/m³。我们对MAB感兴趣的是7个主要的产量QTL和6个容重QTL，其中有一个是共同的，因为它们通过加性效应解释了5%或更多的表型变异。本研究证实了先前鉴定的位点，并鉴定出了新的QTL以及用于提高籽粒产量和农艺性状的有利等位基因。
