Tan Chor-Tee, Yu Hangjin, Yang Yan, Xu Xiangyang, Chen Mingshun, Rudd Jackie C, Xue Qingwu, Ibrahim Amir M H, Garza Lisa, Wang Shichen, Sorrells Mark E, Liu Shuyu
Texas A&M AgriLife Research, Amarillo, TX, 79106, USA.
Department of Soil and Crop Science, Texas A&M University, College Station, TX, 77843, USA.
Theor Appl Genet. 2017 Sep;130(9):1867-1884. doi: 10.1007/s00122-017-2930-4. Epub 2017 Jun 17.
Greenbug and Hessian fly are important pests that decrease wheat production worldwide. We developed and validated breeder-friendly KASP markers for marker-assisted breeding to increase selection efficiency. Greenbug (Schizaphis graminum Rondani) and Hessian fly [Mayetiola destructor (Say)] are two major destructive insect pests of wheat (Triticum aestivum L.) throughout wheat production regions in the USA and worldwide. Greenbug and Hessian fly infestation can significantly reduce grain yield and quality. Breeding for resistance to these two pests using marker-assisted selection (MAS) is the most economical strategy to minimize losses. In this study, doubled haploid lines from the Synthetic W7984 × Opata M85 wheat reference population were used to construct linkage maps for the greenbug resistance gene Gb7 and the Hessian fly resistance gene H32 with genotyping-by-sequencing (GBS) and 90K array-based single nucleotide polymorphism (SNP) marker data. Flanking markers were closely linked to Gb7 and H32 and were located on chromosome 7DL and 3DL, respectively. Gb7-linked markers (synopGBS773 and synopGBS1141) and H32-linked markers (synopGBS901 and IWB65911) were converted into Kompetitive Allele Specific PCR (KASP) assays for MAS in wheat breeding. In addition, comparative mapping identified syntenic regions in Brachypodium distachyon, rice (Oryza sativa), and sorghum (Sorghum bicolor) for Gb7 and H32 that can be used for fine mapping and map-based cloning of the genes. The KASP markers developed in this study are the first set of SNPs tightly linked to Gb7 and H32 and will be very useful for MAS in wheat breeding programs and future genetic studies of greenbug and Hessian fly resistance.
麦二叉蚜和麦秆蝇是降低全球小麦产量的重要害虫。我们开发并验证了对育种者友好的竞争性等位基因特异性PCR（KASP）标记，用于标记辅助育种以提高选择效率。麦二叉蚜（Schizaphis graminum Rondani）和麦秆蝇[Mayetiola destructor (Say)]是美国及全球小麦产区小麦（Triticum aestivum L.）的两种主要毁灭性害虫。麦二叉蚜和麦秆蝇的侵害会显著降低谷物产量和品质。利用标记辅助选择（MAS）培育对这两种害虫的抗性是将损失降至最低的最经济策略。在本研究中，使用来自合成小麦品种W7984×Opata M85小麦参考群体的双单倍体系，通过简化基因组测序（GBS）和基于90K芯片的单核苷酸多态性（SNP）标记数据，构建了抗麦二叉蚜基因Gb7和抗麦秆蝇基因H32的连锁图谱。侧翼标记与Gb7和H32紧密连锁，分别位于7DL染色体和3DL染色体上。与Gb7连锁的标记（synopGBS773和synopGBS1141）以及与H32连锁的标记（synopGBS901和IWB65911）被转化为用于小麦育种MAS的KASP分析。此外，比较作图确定了短柄草、水稻（Oryza sativa）和高粱（Sorghum bicolor）中与Gb7和H32同源的区域，可用于这些基因的精细定位和图位克隆。本研究中开发的KASP标记是与Gb7和H32紧密连锁的首批SNP，将对小麦育种计划中的MAS以及未来麦二叉蚜和麦秆蝇抗性的遗传研究非常有用。
