Abedi Sara, Vessal Mahmood, Asadian Fatemeh, Takhshid Mohammad Ali
Department of Molecular Biology- Biochemistry, Islamic Azad University, Shiraz Branch, Shiraz, Iran.
Laboratory Sciences Department, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran.
J Diabetes Metab Disord. 2021 Sep 12;20(2):1521-1527. doi: 10.1007/s40200-021-00895-z. eCollection 2021 Dec.
The role of indoleamine 2,3-dioxygenase (IDO) has been shown in insulin resistance and metabolic syndrome. The present study aimed to measure serum IDO activity in patients with type 2 diabetes (T2DM) and to determine its association with glycemic control, oxidative stress, and insulin resistance.
Seventy-four patients with T2DM and 74 healthy subjects were selected to participate in this study. Fasting serum biochemical parameters including fasting blood sugar (FBS), HbA1c, insulin, uric acid, albumin, tryptophan, kynurenine, and total antioxidant capacity (TAC) were measured. HOMA-IR, QUICKI, and HOMA-B were calculated using serum FBS and insulin values. IDO activity was estimated using kynurenine/tryptophan ratio (KTR). Data were analyzed using SPSS software (Version 15) and < 0.05 was considered as a significant difference.
The findings showed higher levels of FBS, HbA1c, HOMA-IR, and KTR in the patients compared to the controls TAC and HOMA-B were significantly lowered in the T2DM patients compared to controls. KTR was significantly correlated with the level of HbA1c, and T2DM patients with poor glycemic control (HbA1c ≤ 8) had significantly higher level of KTR. HOMA-B was significantly correlated with serum tryptophan and inversely correlated with HbA1c.
Serum KTR is increased in T2DM patients with poor glycemic control. Potential clinical implications and possible pathogenic roles of IDO in T2DM development should be further elucidated.
吲哚胺2,3-双加氧酶（IDO）在胰岛素抵抗和代谢综合征中的作用已得到证实。本研究旨在测定2型糖尿病（T2DM）患者的血清IDO活性，并确定其与血糖控制、氧化应激和胰岛素抵抗的关系。
选取74例T2DM患者和74例健康受试者参与本研究。测定空腹血清生化参数，包括空腹血糖（FBS）、糖化血红蛋白（HbA1c）、胰岛素、尿酸、白蛋白、色氨酸、犬尿氨酸和总抗氧化能力（TAC）。使用血清FBS和胰岛素值计算稳态模型评估胰岛素抵抗（HOMA-IR）、定量胰岛素敏感性指数（QUICKI）和稳态模型评估胰岛β细胞功能（HOMA-β）。使用犬尿氨酸/色氨酸比值（KTR）估算IDO活性。采用SPSS软件（版本15）进行数据分析，P < 0.05被视为有显著差异。
结果显示，与对照组相比，患者的FBS、HbA1c、HOMA-IR和KTR水平更高；与对照组相比，T2DM患者的TAC和HOMA-β显著降低。KTR与HbA1c水平显著相关，血糖控制不佳（HbA1c ≤ 8）的T2DM患者的KTR水平显著更高。HOMA-β与血清色氨酸显著相关，与HbA1c呈负相关。
血糖控制不佳的T2DM患者血清KTR升高。IDO在T2DM发生发展中的潜在临床意义和可能的致病作用应进一步阐明。
