Kumar Ramesh, Srivastava Priyanshu, Mathur Kalika, Shrinet Jatin, Dubey Sunil Kumar, Chinnappan Mahendran, Kaur Inderjeet, Nayak Debasis, Chattopadhyay Soma, Bhatnagar Raj K, Sunil Sujatha
Vector Borne Diseases Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India.
Discipline of Biosciences and Biomedical Engineering, Indian Institute of Technology Indore, Indore, India.
Virusdisease. 2021 Dec;32(4):657-665. doi: 10.1007/s13337-021-00734-y. Epub 2021 Aug 7.
The non-structural proteins (nsPs) of the chikungunya virus (CHIKV) form the virus's replication complex. They are known to participate in several functions that allow efficient replication of the virus in diverse host systems. One such function is evading the host defense system such as RNA interference (RNAi). Two nsPs of CHIKV, namely, nsP2 and nsP3, were found to suppress the host/vector RNAi machinery and exhibit RNAi suppressor activity. The present study was undertaken to identify interacting partners of CHIKV-nsP3 in . We performed pull-down assays with the mass spectrometry approach and showed the interaction of CHIKV-nsP3 with several proteins. Further co-immunoprecipitation assays revealed that CHIKV-nsP3 interacts with RM62F, a DEAD-box containing RNA known to play roles in multiple gene regulatory processes such as alternative splicing, RNA release, and also is a component of Ago2-RISC complex.
The online version contains supplementary material available at 10.1007/s13337-021-00734-y.
基孔肯雅病毒（CHIKV）的非结构蛋白（nsPs）形成病毒的复制复合体。已知它们参与多种功能，使病毒能够在不同宿主系统中高效复制。其中一种功能是逃避宿主防御系统，如RNA干扰（RNAi）。已发现CHIKV的两种nsP，即nsP2和nsP3，可抑制宿主/载体RNAi机制并表现出RNAi抑制活性。本研究旨在鉴定CHIKV-nsP3在……中的相互作用伙伴。我们采用质谱法进行了下拉实验，结果表明CHIKV-nsP3与多种蛋白质存在相互作用。进一步的免疫共沉淀实验表明，CHIKV-nsP3与RM62F相互作用，RM62F是一种含DEAD框的RNA，已知其在多种基因调控过程中发挥作用，如可变剪接、RNA释放，并且还是Ago2-RISC复合体的一个组分。
在线版本包含可在10.1007/s13337-021-00734-y获取的补充材料。
