Sánchez Martín Darío, Oropesa-Nuñez Reinier, Zardán Gómez de la Torre Teresa
Department of Material Sciences and Engineering, Division of Nanotechnology and Functional Materials, Ångström Laboratory, Uppsala University, 751 21 Uppsala, Sweden.
Department of Material Sciences and Engineering, Division of Solid-State Physics, Ångström Laboratory, Uppsala University, 751 21 Uppsala, Sweden.
ACS Omega. 2021 Nov 23;6(48):32970-32976. doi: 10.1021/acsomega.1c05047. eCollection 2021 Dec 7.
Visual detection of rolling circle amplification products (RCPs) has been achieved by specific aggregation with magnetic nanoparticles. The method presented here reliably generates aggregates in 1.5 h; these are visible to the naked eye in samples containing at least 0.4 fmol of RCPs. In addition, alternate current susceptometry and absorbance spectroscopy have also been used to quantify the amplified products. The specificity of the detection method was tested, and no non-specific aggregation was detected in samples containing up to 20 fmol of non-complementary amplified DNA. This method is a versatile tool for detecting pathogenic DNA in point-of-care diagnostics, with no readout equipment required. However, chips and automated assays can be used in conjugation with the developed method since detection and quantification can be achieved by commercially available readout instruments.
通过与磁性纳米颗粒的特异性聚集实现了滚环扩增产物（RCPs）的视觉检测。此处介绍的方法在1.5小时内可靠地生成聚集体；在含有至少0.4 fmol RCPs的样品中，这些聚集体肉眼可见。此外，还使用了交流感应法和吸收光谱法对扩增产物进行定量。测试了检测方法的特异性，在含有高达20 fmol非互补扩增DNA的样品中未检测到非特异性聚集。该方法是即时诊断中检测致病DNA的通用工具，无需读出设备。然而，芯片和自动化检测可与所开发的方法结合使用，因为检测和定量可通过市售读出仪器实现。
