伊朗伊斯塔乔根结线虫的首次报道。（注：原文中“from istachio”可能有误，推测应该是“from Isfahan”，即来自伊斯法罕，这里按照给定原文翻译）

First report of root-lesion nematode, from istachio in Iran.

作者信息

Naeini Farhad Saeidi, Taheri Zahra Majd

机构信息

Nematology Department, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension, Organization (AREEO), Tehran, Iran.

出版信息

J Nematol. 2021 Dec 8;53. doi: 10.21307/jofnem-2021-103. eCollection 2021.

DOI:10.21307/jofnem-2021-103

PMID:34901873

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8662977/

Abstract

Pistachio, is one of the most important cash crops in Iran that is scattered in arid and semi-arid regions. During a survey of plant-parasitic nematodes of pistachio in Ardakan city in Yazd Province, a species of root-lesion nematode was isolated and identified by morphological, morphometrical, and molecular methods as Palomares-Rius et al., 2014. This species was isolated from several pistachio trees rhizosphere regarding to molecular analysis, D2-D3 expansion segments of 28S rRNA was amplified by PCR and sequenced. The sequence was deposited in GenBank (Accession No. MW338666). Along with the related phylogenetic analysis, placed this species in a monophyletic clade with other isolates, based on Bayesian inference (BI) phylogeny. A PCR reaction with the specific primer set produced a 547 bp fragment. This is the first report of infecting Pistachio tree in the world.

摘要

阿月浑子是伊朗最重要的经济作物之一，分布于干旱和半干旱地区。在对亚兹德省阿尔达坎市阿月浑子的植物寄生线虫进行调查时，通过形态学、形态测量学和分子方法分离并鉴定出一种根腐线虫，该线虫由帕洛马雷斯 - 里乌斯等人于2014年发现。通过分子分析，从几株阿月浑子树的根际分离出该物种，利用PCR扩增28S rRNA的D2 - D3扩展片段并进行测序。该序列已存入GenBank（登录号MW338666）。通过贝叶斯推断（BI）系统发育分析，该物种与其他分离株一起被置于一个单系分支中。使用特异性引物组进行的PCR反应产生了一个547 bp的片段。这是该物种侵染阿月浑子树在世界上的首次报道。