Rodeheaver D P, Wyatt R D, Marks H L
Avian Dis. 1986 Jul-Sep;30(3):568-73.
Serum alpha-amylase (S alpha A) activities were examined in a non-selected population (NS) of Japanese quail and in two lines genetically selected for resistance to aflatoxin. Normal S alpha A activities of both selected lines were significantly lower than that of the NS line (P less than or equal to 0.05). An oral dose of 2.5 mg aflatoxin/kg body weight significantly lowered S alpha A activity in NS quails after 24 hours; 18.0 mg/kg body weight was required to decrease the activity in selected lines to a similar degree. The two selected lines did not differ significantly in response. Disc tube gel electrophoresis of the serum revealed eight bands of S alpha A activity and six S alpha A zymogram phenotypes in the total population using an iodine-staining technique. Neither migration distance nor band width varied with genetic line, but the frequency of quails with at least one of three specific anodic bands was significantly higher in the selected lines (75% and 100%) than in the NS line (27%). The frequencies of the zymogram phenotypes with these bands were similarly increased in the resistant populations. Although there are differences in total S alpha A activity between the selected aflatoxin-resistant lines and the NS line, the presence of specific electrophoretic anodic band types and the S alpha A zymogram may be more useful indicators of aflatoxin resistance.
在一组未经选择的日本鹌鹑群体（NS）以及两个针对黄曲霉毒素抗性进行遗传选择的品系中检测了血清α-淀粉酶（SαA）活性。两个选择品系的正常SαA活性均显著低于NS品系（P≤0.05）。口服2.5毫克/千克体重的黄曲霉毒素24小时后，NS鹌鹑的SαA活性显著降低；而在选择品系中，需要18.0毫克/千克体重才能使活性降低到类似程度。两个选择品系的反应没有显著差异。使用碘染色技术，血清的圆盘管凝胶电泳在整个群体中显示出8条SαA活性带和6种SαA酶谱表型。迁移距离和条带宽度均不随遗传品系而变化，但在选择品系中（75%和100%），至少具有三条特定阳极带之一的鹌鹑频率显著高于NS品系（27%）。在抗性群体中，具有这些条带的酶谱表型频率也同样增加。尽管在选择的抗黄曲霉毒素品系和NS品系之间总SαA活性存在差异，但特定电泳阳极带类型和SαA酶谱的存在可能是更有用的黄曲霉毒素抗性指标。
