State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, 361102, China.
MOE Key Laboratory for Analytical Science of Food Safety and Biology, College of Chemistry, Fuzhou University, Fuzhou, 350108, China.
J Nanobiotechnology. 2021 Dec 13;19(1):419. doi: 10.1186/s12951-021-01168-w.
Accurate diagnosis and effective treatment of primary liver tumors are of great significance, and optical imaging has been widely employed in clinical imaging-guided surgery for liver tumors. The second near-infrared window (NIR-II) emissive AIEgen photosensitizers have attracted a lot of attention with higher-resolution bioimaging and deeper penetration. NIR-II aggregation-induced emission-based luminogen (AIEgen) photosensitizers have better phototherapeutic effects and accuracy of the image-guided surgery/phototherapy. Herein, an NIR-II AIEgen phototheranostic dot was proposed for NIR-II imaging-guided resection surgery and phototherapy for orthotopic hepatic tumors. Compared with indocyanine green (ICG), the AIEgen dots showed bright and sharp NIR-II emission at 1250 nm, which extended to 1600 nm with high photostability. Moreover, the AIEgen dots efficiently generated reactive oxygen species (ROS) for photodynamic therapy. Investigations of orthotopic liver tumors in vitro and in vivo demonstrated that AIEgen dots could be employed both for imaging-guided tumor surgery of early-stage tumors and for 'downstaging' intention to reduce the size. Moreover, the therapeutic strategy induced complete inhibition of orthotopic tumors without recurrence and with few side effects.
准确诊断和有效治疗原发性肝肿瘤具有重要意义，光学成像是肝肿瘤临床影像引导手术中广泛应用的方法。近红外二区（NIR-II）发射的聚集诱导发光（AIE）光敏剂具有更高分辨率的生物成像和更深的穿透能力，受到了广泛关注。基于 NIR-II 聚集诱导发射的发光 AIE 光敏剂具有更好的光疗效果和图像引导手术/光疗的准确性。本研究提出了一种 NIR-II AIE 光热诊疗点，用于 NIR-II 成像引导的原位肝肿瘤切除术和光疗。与吲哚菁绿（ICG）相比，AIE 点在 1250nm 处表现出明亮、锐利的近红外二区发射，其发射光谱可扩展至 1600nm，具有较高的光稳定性。此外，AIE 点还能有效地产生用于光动力治疗的活性氧（ROS）。体外和体内原位肝肿瘤的研究表明，AIE 点既能用于早期肿瘤的成像引导手术，也能用于“降期”治疗以缩小肿瘤体积。此外，该治疗策略诱导了完全抑制原位肿瘤，无复发，且副作用少。
