Lundberg C, Marceau F, Huey R, Hugli T E
Immunopharmacology. 1986 Oct;12(2):135-43. doi: 10.1016/0162-3109(86)90039-1.
Subcultured endothelial cells from human umbilical veins respond to histamine and melittin with increased prostacyclin production, measured as 6-keto-prostaglandin F1 alpha by radioimmunoassay. However, no response to leukotriene C4 was observed. Primary cultured cells, on the other hand, respond to leukotriene C4 and the histamine response was 7 times more potent for these cells than for subcultured cells. In contrast, neither primary cultures nor subcultures of endothelial cells released prostacyclin following application of either human anaphylatoxin C5a (100 nM) or C3a (1 microM). In addition, these endothelial cells appear to have no specific binding sites for 125I-C5a. However, endothelial cells released prostacyclin in the presence of human polymorphonuclear leukocytes that were activated with C5a. We conclude that involvement of endothelial cells in the haemodynamic response to anaphylatoxin is an indirect function, i.e. C5a activates circulating or tissue cells which in turn stimulate the endothelial cell to produce prostacyclin.
用人脐静脉传代培养的内皮细胞对组胺和蜂毒肽有反应，前列腺素生成增加，通过放射免疫测定法测定为6-酮-前列腺素F1α。然而，未观察到对白三烯C4的反应。另一方面，原代培养细胞对白三烯C4有反应，并且这些细胞对组胺的反应比对传代培养细胞的反应强7倍。相反，内皮细胞的原代培养物和传代培养物在应用人过敏毒素C5a（100 nM）或C3a（1 μM）后均未释放前列腺素。此外，这些内皮细胞似乎没有125I-C5a的特异性结合位点。然而，内皮细胞在被C5a激活的人多形核白细胞存在的情况下释放前列腺素。我们得出结论，内皮细胞参与对过敏毒素的血流动力学反应是一种间接功能，即C5a激活循环或组织细胞，进而刺激内皮细胞产生前列腺素。
