Martinez-Sales V, Gomez-Lechón M J, Gilabert J
Research Center, Hospital La Fe, Valencia, Spain.
Cytotechnology. 1990 Jan;3(1):21-9. doi: 10.1007/BF00365262.
Human umbilical endothelial cells in culture retain differentiated morphological and functional characterization in primary culture and even in the early subcultures, after which they begin to degenerate. We have studied the morphological and biochemical characterization (ability to produce prostacyclin, prostaglandin E2 and thromboxane A2 in culture) of endothelial cells in the first seven subcultures. In addition the influence of serum and endothelial cell growth factor added to the culture medium have been evaluated. With 20% normal human serum, cell proliferation is faster than with the same concentration of human fetal or bovine fetal serum. After the 3rd passage, morphological and growth alterations become observable in the endothelial cells. However, prostacyclin, prostaglandin E2 and thromboxane A2 production showed no variations during the study.
培养中的人脐内皮细胞在原代培养甚至早期传代培养时仍保留分化的形态和功能特征，此后它们开始退化。我们研究了前七代传代培养的内皮细胞的形态和生化特征（在培养中产生前列环素、前列腺素E2和血栓素A2的能力）。此外，还评估了添加到培养基中的血清和内皮细胞生长因子的影响。使用20%正常人血清时，细胞增殖比使用相同浓度的人胎血清或牛胎血清时更快。在第3代传代后，内皮细胞中可观察到形态和生长变化。然而，在研究期间，前列环素、前列腺素E2和血栓素A2的产生没有变化。
