Vijaya S, Steffen D L, Robinson H L
J Virol. 1986 Nov;60(2):683-92. doi: 10.1128/JVI.60.2.683-692.1986.
Seven cellular loci with acceptor sites for retroviral integrations have been mapped for the presence of DNase I-hypersensitive sites in chromatin. Integrations in three of these loci, chicken c-erbB, rat c-myc, and a rat locus, dsi-1, had been selected for in retrovirus-induced tumors. Of the remaining four, two, designated dsi-3 and dsi-4, harbored acceptor sites for apparently unselected integrations of Moloney murine leukemia virus in a Moloney murine leukemia virus-induced thymoma, and two, designated C and F, harbored unselected acceptor sites for Moloney murine leukemia virus integrations in a rat fibroblast cell line. Each acceptor site mapped to within 500 base pairs of a DNase I-hypersensitive site. In the analyses of the unselected integrations, six hypersensitive sites were observed in 39 kilobases of DNA. The four acceptor sites in this DNA were localized between 0.05 and 0.43 kilobases of a hypersensitive site. The probability of this close association occurring by chance was calculated to be extremely low. Hypersensitive sites were mapped in cells representing the lineage in which integration had occurred as well as in an unrelated lineage. In six of the seven acceptor loci hypersensitive sites could not be detected in the unrelated lineage. Our results indicate that retroviruses preferentially integrate close to DNase I-hypersensitive sites and that many of these sites are expressed in some but not all cells.
已对七个具有逆转录病毒整合受体位点的细胞位点进行染色质中DNase I超敏位点的定位。在逆转录病毒诱导的肿瘤中，已筛选出其中三个位点（鸡c-erbB、大鼠c-myc和一个大鼠位点dsi-1）的整合情况。在其余四个位点中，有两个位点（分别命名为dsi-3和dsi-4）在莫洛尼氏鼠白血病病毒诱导的胸腺瘤中含有莫洛尼氏鼠白血病病毒明显未被筛选的整合受体位点，另外两个位点（分别命名为C和F）在大鼠成纤维细胞系中含有莫洛尼氏鼠白血病病毒未被筛选的整合受体位点。每个受体位点都定位在DNase I超敏位点的500个碱基对内。在对未被筛选的整合情况进行分析时，在39千碱基的DNA中观察到六个超敏位点。该DNA中的四个受体位点定位在超敏位点的0.05至0.43千碱基之间。经计算，这种紧密关联偶然发生的概率极低。在代表整合发生谱系的细胞以及无关谱系的细胞中都对超敏位点进行了定位。在七个受体位点中的六个位点，在无关谱系中未检测到超敏位点。我们的结果表明，逆转录病毒优先整合在接近DNase I超敏位点的位置，并且这些位点中的许多在某些细胞而非所有细胞中表达。
