State Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Nanchang University, 235 Nanjing East Road, Nanchang 330047, China.
State Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Nanchang University, 235 Nanjing East Road, Nanchang 330047, China.
Food Chem. 2022 Apr 16;374:131508. doi: 10.1016/j.foodchem.2021.131508. Epub 2021 Nov 24.
This work established an effective method for kale flavonoids enrichment by resins. Resin screening, adsorption kinetics and isotherms, dynamic adsorption and desorption tests were conducted to optimize the appropriate resins and enrichment conditions. The results showed that NKA-9 was the optimum resin. The best adsorption conditions were 0.2 mg/mL flavonoids concentration, 12.5 bed volume (BV) sample volume and 2 BV/h adsorption rate. The desorption conditions were 3 BV of 80% ethanol at 2 BV/h elution rate. Under these conditions, the product purity was 31.16%. The purified flavonoids extract was mainly comprised of Kaempferol-3-O-sophoroside-7-O-diglucoside, Kaempferol-3,7,4'-O-d-triglucoside, Kaempferol-3-O-feruloyl-sophoroside-7-O-d-glucoside, and Kaempferol-3-O-sinapoyl-sophoroside. Moreover, it presented higher scavenging ability against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and hydroxyl (OH) radical than crude extract. In conclusion, kale flavonoids can be well concentrated by NKA-9 resin and the purified flavonoids extract has good antioxidant activity which can be potentially applied in food, cosmetic or pharmacy industries.
本研究建立了一种有效的树脂法从羽衣甘蓝中富集类黄酮的方法。通过树脂筛选、吸附动力学和等温线、动态吸附和解吸试验，优化了合适的树脂和富集条件。结果表明，NKA-9 是最佳树脂。最佳吸附条件为黄酮浓度 0.2mg/mL、样品体积 12.5 床体积（BV）和吸附速率 2BV/h。最佳解吸条件为 80%乙醇 3BV，洗脱速率 2BV/h。在此条件下，产品纯度为 31.16%。纯化的类黄酮提取物主要由山柰酚-3-O-槐糖苷-7-O-双葡萄糖苷、山柰酚-3,7,4'-O-三葡萄糖苷、山柰酚-3-O-阿魏酰槐糖苷-7-O-葡萄糖苷和山柰酚-3-O-芥子酰槐糖苷组成。此外，它对 1,1-二苯基-2-苦基肼（DPPH）自由基和羟基（OH）自由基的清除能力均高于粗提物。总之，NKA-9 树脂可以很好地浓缩羽衣甘蓝类黄酮，纯化的类黄酮提取物具有良好的抗氧化活性，可潜在应用于食品、化妆品或制药行业。
