College of Biological and Pharmaceutical Engineering, Jilin Agricultural Science and Technology University, Jilin, PR China/College of Veterinary Medicine, Northeast Agricultural University, Harbin, PR China.
College of Biological and Pharmaceutical Engineering, Jilin Agricultural Science and Technology University, Jilin, PR China.
Pak J Pharm Sci. 2024 Jul;37(4):881-890.
This study aimed to enrich flavonoids from Euphorbia hirta L. (E. hirta) extracts, and the enrichment parameters were optimized by adsorption and desorption tests. The HPD-300 resin was chosen after a comparison of the flavonoids from E. hir15ta's adsorption and desorption capabilities on nine different types of macro porous resin. The optimal enrichment for purification of E. hirta extracts were determined as sample concentration of 3.0mg/mL, pH of 2.0 and a desorption solvent of 50% ethanol. The optimal dynamic parameters were loading 2.5 BV of sample at a feeding flow rate of 2 BV/h, cleaning the column with 5 BV of water and then eluting 50.0% ethanol at a 2 BV/h elution flow rate using 5 BV of eluent. Following a single treatment cycle with HPD-300 resin, the product's total flavonoid content rose from 6.32% to 28.8%, with an 80.01% recovery yield. Then, 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) and hydroxyl radical scavenging ability were used to evaluate the antioxidant properties of the purified flavonoids. The main chemical components of purified flavonoids from E. hirta are astragalin, quercetin-3β-D-glucoside, 9,16-dioxo-10,12,14-octadeca-trienoic acid and gallic acid. The results showed that purified flavonoids from E. hirta had a strong antioxidant effect, which indicated that it represented a valuable natural antioxidant source.
本研究旨在从大戟属植物(Euphorbia hirta L.)提取物中富集黄酮类化合物，并通过吸附和解吸试验对富集参数进行优化。在比较了 9 种不同类型大孔树脂对大戟属植物吸附和解吸能力后，选择 HPD-300 树脂。确定最佳富集条件为：样品浓度 3.0mg/mL、pH 值 2.0、洗脱剂为 50%乙醇。最佳动态参数为：上样流速 2BV/h，上样 2.5BV，水洗 5BV，50%乙醇洗脱流速 2BV/h，洗脱 5BV。经 HPD-300 树脂处理后，产品总黄酮含量从 6.32%提高到 28.8%，回收率为 80.01%。然后，采用 1,1-二苯基-2-苦基肼基（DPPH）、2,2'-联氮-二（3-乙基苯并噻唑啉-6-磺酸）（ABTS）和羟基自由基清除能力评价纯化黄酮类化合物的抗氧化性能。大戟属植物纯化黄酮类化合物的主要化学成分有：毛蕊异黄酮、槲皮素-3β-D-葡萄糖苷、9,16-二酮基-10,12,14-十八碳三烯酸和没食子酸。结果表明，大戟属植物纯化黄酮类化合物具有较强的抗氧化作用，表明其是一种有价值的天然抗氧化剂来源。
