College of Resources and Environment, Key Laboratory of Agricultural Environment, Shandong Agricultural University, Tai'an 271018, PR China.
College of Resources and Environment, Key Laboratory of Agricultural Environment, Shandong Agricultural University, Tai'an 271018, PR China.
J Hazard Mater. 2022 Mar 5;425:128027. doi: 10.1016/j.jhazmat.2021.128027. Epub 2021 Dec 8.
Dibutyl phthalate (DBP), one of the most commonly applied plasticizers, has been frequently detected in the aquatic environment, posing potential risks to aquatic organisms. Currently, reports about the toxicity of zebrafish liver with DBP exposure are rare, and the toxic mechanism is still not clear. In this study, zebrafish (Danio rerio) were used to explore the ecotoxicological effects of DBP from the physiological, biochemical, genetic, and molecular levels. The results showed oxidative stress, lipid peroxidation, and DNA damage occurred in zebrafish liver according to changes in antioxidant enzymes, MDA and 8-OHdG content. AchE activity was always active, and negatively correlated with the DBP concentration. The expression of Cu/Zn-sod and gpx genes were similar to that of antioxidant enzymes from 7 to 21 days, while in the end, the inconsistent result appeared due to the time lag effect in protein modification, gene transcription and translation. Besides, the mRNA abundance of Caspase-3 and p53 were upregulated, showing a "dose-response" relationship. The integrated biomarker reaction indicated that the effects of exposure time on zebrafish liver was 14th day> 28th day> 7th day> 21th day. These results are of great significance to evaluate the toxicological effects and explore the toxic mechanism of DBP on aquatic organisms.
邻苯二甲酸二丁酯（DBP）是最常用的增塑剂之一，经常在水环境中被检测到，对水生生物构成潜在风险。目前，关于 DBP 暴露对斑马鱼肝脏毒性的报道很少，其毒性机制尚不清楚。本研究采用斑马鱼（Danio rerio）从生理、生化、遗传和分子水平探讨 DBP 的生态毒理学效应。结果表明，抗氧化酶、MDA 和 8-OHdG 含量的变化导致斑马鱼肝脏发生氧化应激、脂质过氧化和 DNA 损伤。乙酰胆碱酯酶（AchE）活性一直保持活跃，并与 DBP 浓度呈负相关。Cu/Zn-sod 和 gpx 基因的表达与抗氧化酶相似，持续到第 7 天至第 21 天，而在蛋白质修饰、基因转录和翻译的时间滞后效应作用下，最终出现不一致的结果。此外，Caspase-3 和 p53 的 mRNA 丰度上调，呈现“剂量-反应”关系。综合生物标志物反应表明，暴露时间对斑马鱼肝脏的影响为 14 天>28 天>7 天>21 天。这些结果对评估 DBP 对水生生物的毒理学效应和探讨其毒性机制具有重要意义。
