Dewar C
J Immunol Methods. 1978;20:301-10. doi: 10.1016/0022-1759(78)90265-x.
A method is described by which granulocytes may be isolated from human peripheral blood. The yield of granulocytes using this technique (70.9%) is considerably greater than that obtained by alternative methods. The isolated cells are mainly composed of neutrophils, but the ratio of eosinophils to neutrophils is not significantly different from that of the peripheral blood. The cells are functionally comparable to buffy coat granulocytes with respect to phagocytic, candidacidal and NBT dye-reduction ability. Removal of erythrocytes from the final cells gives a granulocyte preparation useful for studies in which purity is essential.
本文描述了一种从人外周血中分离粒细胞的方法。使用该技术分离粒细胞的产量（70.9%）显著高于其他替代方法。分离出的细胞主要由中性粒细胞组成，但嗜酸性粒细胞与中性粒细胞的比例与外周血中的比例无显著差异。就吞噬、杀念珠菌和NBT染料还原能力而言，这些细胞在功能上与血沉棕黄层粒细胞相当。从最终细胞中去除红细胞可得到适用于纯度至关重要的研究的粒细胞制剂。
