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氯化铵处理对人多形核白细胞碘化作用的影响。

Effect of ammonium chloride treatment on human polymorphonuclear leucocyte iodination.

作者信息

Phillips W A, Hosking C S, Shelton M J

出版信息

J Clin Pathol. 1983 Jul;36(7):808-10. doi: 10.1136/jcp.36.7.808.

DOI:10.1136/jcp.36.7.808
PMID:6863573
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC498393/
Abstract

A discontinuous gradient of Percoll was used to remove RBCs from polymorphonuclear leucocyte (PMN) preparations. The resulting red blood cell-free preparation was used to investigate the effect of ammonium chloride on the iodination response of human PMN. Treatment of PMN with ammonium chloride for five minutes at room temperature resulted in a statistically significant increase in the iodination response of both resting and stimulated PMN.

摘要

使用不连续的 Percoll 梯度从多形核白细胞（PMN）制剂中去除红细胞。所得无红细胞制剂用于研究氯化铵对人 PMN 碘化反应的影响。在室温下用氯化铵处理 PMN 五分钟，导致静息和刺激的 PMN 的碘化反应均有统计学上的显著增加。

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本文引用的文献

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Separation of granulocytes from peripheral blood in a single step using discontinuous density gradients of Ficoll-Urografin. A comparative study with separation by dextran.使用菲可-泛影葡胺不连续密度梯度一步从外周血中分离粒细胞。与右旋糖酐分离法的比较研究。
J Immunol Methods. 1980;32(1):41-50. doi: 10.1016/0022-1759(80)90115-5.
2
Macrophage antimicrobial activity: evidence for participation by lysosomes in the killing of Saccharomyces cerevisiae by normal resident macrophages.巨噬细胞的抗菌活性：溶酶体参与正常驻留巨噬细胞杀灭酿酒酵母的证据。
Infect Immun. 1981 Feb;31(2):828-30. doi: 10.1128/iai.31.2.828-830.1981.
3
An extension of the 51Cr-release assay for the estimation of mouse cytotoxins.用于估计小鼠细胞毒素的51铬释放试验的扩展。
Transplantation. 1968 Sep;6(6):761-4. doi: 10.1097/00007890-196809000-00002.
4
The separation of different cell classes from lymphoid organs. V. Simple procedures for the removal of cell debris. Damaged cells and erythroid cells from lymphoid cell suspensions.从淋巴器官中分离不同细胞类别。五、去除细胞碎片的简单程序。淋巴样细胞悬液中的受损细胞和红细胞。
J Immunol Methods. 1972 May;1(3):273-87. doi: 10.1016/0022-1759(72)90005-1.
5
Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g.从人血中分离单核细胞和粒细胞。通过一次离心分离单核细胞，通过离心和1g沉降相结合的方法分离粒细胞。
Scand J Clin Lab Invest Suppl. 1968;97:77-89.
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An improved method for isolation of granulocytes from peripheral blood.一种从外周血中分离粒细胞的改良方法。
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