De Dios I, Manso M, Leon V, Lopez Borrasca A
Biochem Med Metab Biol. 1986 Feb;35(1):12-7. doi: 10.1016/0885-4505(86)90053-8.
The specificity and affinity of eight lectins (concanavalin A, L. culinaris, P. sativum, phytohemagglutinin P, D. biflorus, soybean agglutinin, T. purpureus, and T. vulgaris) to B, T, T gamma, and T mu lymphocytes from the blood of normal subjects were determined. Lectins attached to latex particles were used to evaluate the binding of each lectin to individual cells. The rosette percentage found in each lymphocyte population expresses the specificity index and the specific sugar concentrations needed to decrease the rosette percentage by 50% is taken as the affinity index. B Lymphocytes showed a major subclass, with respect to T lymphocytes, with receptors for WGA, SBA, D. biflorus, L. culinaris, and P. sativum lectins. In contrast, T lymphocytes exhibit a greater number of cells with specific receptors for Con A, T. purpureus, and PHA lectins than B lymphocytes, the T gamma subpopulation being responsible for the specificity of the first two lectins and the T mu subpopulation for the PHA lectin.
测定了八种凝集素（刀豆球蛋白A、菜豆凝集素、豌豆凝集素、植物血凝素P、双花扁豆凝集素、大豆凝集素、紫穗槐凝集素和普通小麦凝集素）对正常受试者血液中B淋巴细胞、T淋巴细胞、Tγ淋巴细胞和Tμ淋巴细胞的特异性和亲和力。附着在乳胶颗粒上的凝集素用于评估每种凝集素与单个细胞的结合。在每个淋巴细胞群体中发现的花环百分比表示特异性指数，使花环百分比降低50%所需的特定糖浓度作为亲和力指数。与T淋巴细胞相比，B淋巴细胞显示出一个主要亚类，具有与麦胚凝集素、大豆凝集素、双花扁豆凝集素、菜豆凝集素和豌豆凝集素结合的受体。相反，T淋巴细胞中具有与刀豆球蛋白A、紫穗槐凝集素和植物血凝素凝集素特异性受体的细胞数量比B淋巴细胞更多，Tγ亚群负责前两种凝集素的特异性，Tμ亚群负责植物血凝素凝集素的特异性。
