Whitehurst C E, Day N K, Gengozian N
Department of Pediatrics, All Children's Hospital, University of South Florida, St. Petersburg 33701.
J Immunol Methods. 1994 Oct 14;175(2):189-99. doi: 10.1016/0022-1759(94)90362-x.
The binding properties of several plant lectins on feline peripheral blood lymphocytes (PBL) were examined by flow cytometry to reveal if any could serve as probes for identifying specific lymphocyte subclasses. Most of the lectins bound > or = 90% of PBL in a uniform manner. The exceptions were the lectins from Glycine max, Phaseolus limensis, and Dolichos biflorus, which bound only a proportion of the PBL. The differential binding of the lectins to lymphocyte subclasses was examined by comparing the staining of control PBL to T cell-depleted PBL. The lectins from Pisum sativum, Lens culinaris, succinylated Triticum vulgaris, and Dolichos biflorus stained T-depleted PBL more brightly than control PBL. Extensive cytofluorometric analyses of the binding properties of Pisum sativum agglutinin (PSA) on feline PBL and mesenteric lymph node cells indicated that feline B lymphocytes express more higher affinity surface receptors for PSA than do T lymphocytes. The higher affinity binding of PSA to B lymphocytes was further demonstrated by PSA-mediated cellular affinity chromatography. With this procedure it was possible to deplete PBL of B lymphocytes and to obtain pure populations of feline T lymphocytes.
通过流式细胞术检测了几种植物凝集素对猫外周血淋巴细胞（PBL）的结合特性，以确定是否有凝集素可作为识别特定淋巴细胞亚群的探针。大多数凝集素以均匀的方式结合≥90%的PBL。例外的是来自大豆、利马豆和双花扁豆的凝集素，它们只结合一部分PBL。通过比较对照PBL与T细胞耗竭的PBL的染色情况，检测了凝集素与淋巴细胞亚群的差异结合。来自豌豆、兵豆、琥珀酰化小麦和双花扁豆的凝集素对T细胞耗竭的PBL的染色比对照PBL更亮。对豌豆凝集素（PSA）与猫PBL和肠系膜淋巴结细胞的结合特性进行的广泛细胞荧光分析表明，猫B淋巴细胞比T淋巴细胞表达更多对PSA具有更高亲和力的表面受体。PSA介导的细胞亲和层析进一步证明了PSA与B淋巴细胞的高亲和力结合。通过该方法，可以去除PBL中的B淋巴细胞，并获得猫T淋巴细胞的纯群体。
