Nomoto Y, Suga T, Miura M, Nomoto H, Tomino Y, Sakai H
Clin Exp Immunol. 1986 Sep;65(3):513-9.
A nuclear antigen is recognized by autoantibodies in the sera of some patients with IgA nephropathy. Using these autoantibodies as a reagent, this antigen was purified 77.2-fold by ammonium sulfate fractionation, DEAE chromatography and Sepharose 6BCL gel filtration. The antigenicity of this antigen was sensitive to trypsin but resistant to RNase and DNase, suggesting that the antigenic determinant resided in protein and not nucleic acids. This antigen was inactivated at 56 degrees C for 3 h. Isoelectrophoretic focussing showed that the pI was below 4. The immunoblotting (Western transfer) assay showed a single polypeptide (69,000 Daltons) which proved to be a reactive antigen.
在一些IgA肾病患者的血清中，自身抗体可识别一种核抗原。以这些自身抗体作为试剂，通过硫酸铵分级分离、DEAE柱层析和琼脂糖6BCL凝胶过滤，该抗原被纯化了77.2倍。该抗原的抗原性对胰蛋白酶敏感，但对核糖核酸酶和脱氧核糖核酸酶有抗性，这表明抗原决定簇存在于蛋白质而非核酸中。该抗原在56℃下3小时会失活。等电聚焦显示其等电点低于4。免疫印迹（Western印迹）分析显示有一条单一的多肽（69,000道尔顿），它被证明是一种反应性抗原。
